EC Number | General Stability | Organism |
---|---|---|
3.2.1.135 | 2 mM EDTA and 2 mM phytic acid decreases the stability of the enzyme | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.135 | Ca2+ | 5 mM Ca2+ increases the stability of the enzyme | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.135 | 100000 | - |
1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.135 | Geobacillus stearothermophilus | - |
- |
- |
3.2.1.135 | Geobacillus stearothermophilus L14 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.135 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.135 | maltoheptaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | maltoheptaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
3.2.1.135 | maltohexaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | maltohexaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
3.2.1.135 | maltopentaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | maltopentaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
3.2.1.135 | maltotetraose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | maltotetraose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
3.2.1.135 | maltotriose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | additional information | the enzyme is unable to degrade alpha-cyclodextrin and panose | Geobacillus stearothermophilus | ? | - |
? | |
3.2.1.135 | additional information | the enzyme is unable to degrade alpha-cyclodextrin and panose | Geobacillus stearothermophilus L14 | ? | - |
? | |
3.2.1.135 | pullulan + H2O | the enzyme attacks both alpha-D-(1->4) and alpha-D-(1->6) glycosidic linkages | Geobacillus stearothermophilus | D-glucose + maltose + maltotriose | - |
? | |
3.2.1.135 | soluble starch + H2O | - |
Geobacillus stearothermophilus | D-glucose + maltose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.135 | monomer | 1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.135 | amylopullulanase | - |
Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.135 | 65 | - |
- |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.135 | 60 | 70 | the irreversible thermoinactivation of the enzyme is recorded in 50 mM sodium acetate, pH 5.5 at 60, 70 and 80°C. Pullulanase retains 85% of its activity after 50 min of incubation at 60°C, its residual activities at 70 and 80°C after 50 min are 70 and 15%, respectively | Geobacillus stearothermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.135 | 62000 | - |
pullulan | - |
Geobacillus stearothermophilus | |
3.2.1.135 | 111000 | - |
soluble starch | - |
Geobacillus stearothermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.135 | 5.5 | - |
- |
Geobacillus stearothermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.135 | 5 | 7 | - |
Geobacillus stearothermophilus |