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Literature summary extracted from

  • Sullivan, L.; Cates, M.; Bennett, G.
    Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes (2010), Enzyme Microb. Technol., 46, 118-124.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.7 E332D the mutant shows 3.82% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 E332L the mutant shows 0.34% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 E332Q the mutant shows 3.02% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 E334D the mutant shows 0.29% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 E334L the mutant shows0.58% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 E334Q the mutant shows 0.41% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 H155L the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 H155Q the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 H183L the mutant shows 0.06% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 H183Q the mutant shows 0.19% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 R150F the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 R150K the mutant shows 21% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 R215F E332D the mutant shows 0.38% of wild type butyrate kinase II activity Clostridium acetobutylicum
2.7.2.7 R215K the mutant shows 0.44% of wild type butyrate kinase II activity Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.7 42000
-
 SDS-PAGE Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.7 Clostridium acetobutylicum
-
 strain ATCC 824
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.7 nickel affinity column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.7 ATP + butanoate
-
 Clostridium acetobutylicum ADP + butanoyl phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.2.7 BKI isozyme Clostridium acetobutylicum
2.7.2.7 BKII isozyme Clostridium acetobutylicum
2.7.2.7 butyrate kinase I isozyme Clostridium acetobutylicum
2.7.2.7 butyrate kinase II isozyme Clostridium acetobutylicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.7 ATP
-
 Clostridium acetobutylicum