BRENDA - Enzyme Database

Lecithin: cholesterol acyltransferase - from biochemistry to role in cardiovascular disease

Rousset, X.; Vaisman, B.; Amar, M.; Sethi, A.; Remaley, A.; Curr. Opin. Endocrinol. Diabetes Obes. 16, 163-171 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.43
apolipoprotein A-I
required, other apolipoprotein are less effective
Homo sapiens
2.3.1.43
apolipoprotein A-I
required
Mus musculus
2.3.1.43
apolipoprotein A-I
required
Oryctolagus cuniculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.43
additional information
enzyme deficiency phenotypes, overview. Generation of transgenic mice, monkeys, or rabbits overexpressing the human LCAT
Homo sapiens
2.3.1.43
additional information
overexpression of LCAT does not protect against diet-induced atherosclerosis, but in contrary does increase the atherosclerosis risk. LCAT knockout mice show markedly reduced plasma total cholesterol, cholesteryl esters, HDL-C, apolipoprotein A-I, and an increase in plasma triglycerides, the amount of alpha-HDl is decreased compared to wild-type mice
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.43
sphingomyelin
-
Homo sapiens
2.3.1.43
sphingomyelin
-
Mus musculus
2.3.1.43
sphingomyelin
-
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.43
extracellular
-
Mus musculus
-
-
2.3.1.43
extracellular
-
Oryctolagus cuniculus
-
-
2.3.1.43
extracellular
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.43
additional information
Homo sapiens
about 75% of plasma LCAT is associated with HDL
?
-
-
-
2.3.1.43
phosphatidylcholine + cholesterol
Mus musculus
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
Homo sapiens
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
Oryctolagus cuniculus
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.43
Homo sapiens
-
gene lcat
-
2.3.1.43
Mus musculus
-
gene lcat
-
2.3.1.43
Oryctolagus cuniculus
-
gene lcat
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.43
blood plasma
about 75% of plasma LCAT is associated with HDL
Homo sapiens
-
2.3.1.43
blood plasma
-
Oryctolagus cuniculus
-
2.3.1.43
blood plasma
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.43
additional information
about 75% of plasma LCAT is associated with HDL
703346
Homo sapiens
?
-
-
-
-
2.3.1.43
additional information
the human enzyme prefers phospholipids with 18:1 or 18:2 fatty acids
703346
Homo sapiens
?
-
-
-
-
2.3.1.43
additional information
the mouse enzyme prefers phospholipids with 20:4 fatty acids
703346
Mus musculus
?
-
-
-
-
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Mus musculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Homo sapiens
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Mus musculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Homo sapiens
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Mus musculus
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Homo sapiens
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.43
apolipoprotein A-I
required, other apolipoprotein are less effective
Homo sapiens
2.3.1.43
apolipoprotein A-I
required
Mus musculus
2.3.1.43
apolipoprotein A-I
required
Oryctolagus cuniculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.43
additional information
enzyme deficiency phenotypes, overview. Generation of transgenic mice, monkeys, or rabbits overexpressing the human LCAT
Homo sapiens
2.3.1.43
additional information
overexpression of LCAT does not protect against diet-induced atherosclerosis, but in contrary does increase the atherosclerosis risk. LCAT knockout mice show markedly reduced plasma total cholesterol, cholesteryl esters, HDL-C, apolipoprotein A-I, and an increase in plasma triglycerides, the amount of alpha-HDl is decreased compared to wild-type mice
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.43
sphingomyelin
-
Homo sapiens
2.3.1.43
sphingomyelin
-
Mus musculus
2.3.1.43
sphingomyelin
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.43
extracellular
-
Mus musculus
-
-
2.3.1.43
extracellular
-
Oryctolagus cuniculus
-
-
2.3.1.43
extracellular
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.43
additional information
Homo sapiens
about 75% of plasma LCAT is associated with HDL
?
-
-
-
2.3.1.43
phosphatidylcholine + cholesterol
Mus musculus
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
Homo sapiens
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
Oryctolagus cuniculus
-
cholesteryl ester + lysophosphatidylcholine
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.43
blood plasma
about 75% of plasma LCAT is associated with HDL
Homo sapiens
-
2.3.1.43
blood plasma
-
Oryctolagus cuniculus
-
2.3.1.43
blood plasma
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.43
additional information
about 75% of plasma LCAT is associated with HDL
703346
Homo sapiens
?
-
-
-
-
2.3.1.43
additional information
the human enzyme prefers phospholipids with 18:1 or 18:2 fatty acids
703346
Homo sapiens
?
-
-
-
-
2.3.1.43
additional information
the mouse enzyme prefers phospholipids with 20:4 fatty acids
703346
Mus musculus
?
-
-
-
-
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Mus musculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Homo sapiens
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
-
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Mus musculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Homo sapiens
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylcholine + cholesterol
in a first step, LCAT hydrolyzes the sn-2 acyl group of phosphatidylcholine and binds it at Ser181. In a second step, the enzyme transfers and esterifies the fatty acid to the 3beta-hydroxyl group on the A-ring of free cholesterol to form cholesteryl ester using apolipoprotein A-I as cofactor
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylcholine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Mus musculus
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Homo sapiens
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
2.3.1.43
phosphatidylethanolamine + cholesterol
-
703346
Oryctolagus cuniculus
cholesteryl ester + lysophosphatidylethanolamine
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.3.1.43
malfunction
inborn enzyme deficiency leads to the fish-eye disease, as well as anemia, proteinuria, renal failure, hepatosplenomegaly, and lymphadenopathy
Homo sapiens
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Homo sapiens
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Mus musculus
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Oryctolagus cuniculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.43
malfunction
inborn enzyme deficiency leads to the fish-eye disease, as well as anemia, proteinuria, renal failure, hepatosplenomegaly, and lymphadenopathy
Homo sapiens
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Homo sapiens
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Mus musculus
2.3.1.43
physiological function
LCAT might have a beneficial role in reducing atherosclerosis
Oryctolagus cuniculus