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Literature summary extracted from

  • Svedruzic, Z.M.
    Mammalian cytosine DNA methyltransferase Dnmt1: enzymatic mechanism, novel mechanism-based inhibitors, and RNA-directed DNA methylation (2008), Curr. Med. Chem., 15, 92-106.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 2-pyrimidinone forms a part of inhibitor 1,2-dihydropyrimidin-2-one-5-methylene-(methylsulfonium)-adenosyl, bindng structure and mechanism, overview Homo sapiens
2.1.1.37 5-aza-cytosine
-
 Homo sapiens
2.1.1.37 5-fluorocytosine
-
 Homo sapiens
2.1.1.37 [1,2-dihydropyrimidin-2-one]-5-methylene-(methylsulfonium)-adenosyl with 2-pyrimidinone ring Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 additional information
-
 additional information Michaelis-Menten kinetics, Dnmt1 allosteric regulation mechanism, modelling, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.37 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.37 DNA + S-adenosyl-L-methionine Homo sapiens
-
 DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Homo sapiens P26358
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.37 S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine catalytic reaction mechanism of Dnmt1, allosteric regulation with fast equilibrium, and substrate specificity. Base-flipping and base-stacking are the key mechanism in control of enzymatic activity, detailed overview Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 DNA + S-adenosyl-L-methionine
-
 Homo sapiens DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?
2.1.1.37 DNA + S-adenosyl-L-methionine Dnmt1 methylates DNA at CG sites. Substrate binding and involved motifs in Dnmt1, overview Homo sapiens DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?
2.1.1.37 additional information no activity with single-stranded DNA with one Cm5 site, double-stranded fully methylated DNA, and poly(dA)-poly(T), pol(G), poly(dC)-poly(dG) or poly(dA-dT) Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.37 More Dnmt1 has a small catalytid C-terminal domain and a large regulatory domain at the N-terminal, the two domains are linked by 12 Gly-Lys repeats, structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.37 cytosine carbon 5 DNA methyltransferase
-
 Homo sapiens
2.1.1.37 cytosine DNA methyltransferase
-
 Homo sapiens
2.1.1.37 Dnmt1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.37 S-adenosyl-L-methionine
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.37 additional information
-
 additional information inhibition by mechanism-based inhibitors and kinetics, detailed overview Homo sapiens