BRENDA - Enzyme Database

Exploring the substrate promiscuity of drug-modifying enzymes for the chemoenzymatic generation of N-acylated aminoglycosides

Green, K.D.; Chen, W.; Houghton, J.L.; Fridman, M.; Garneau-Tsodikova, S.; ChemBioChem 11, 119-126 (2010)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.3.1.81
synthesis
development of a methodology that utilizes aminoglycoside acetyltransferase and unnatural acyl-CoA analogues for the chemoenzymatic generation of regioselectively N-acylatedaminoglycoside analogues. Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections
Escherichia coli
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.81
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.81
0.00057
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.00096
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0015
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.002
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.0021
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0027
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0029
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0031
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0033
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0046
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0048
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.0054
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0055
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0091
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0099
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0111
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0135
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0148
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0189
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0716
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.81
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + amikacin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
6-fluoropicolinyl-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
acetyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-acetyl-amikacin
-
-
-
?
2.3.1.81
acetyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-acetyl-gentamicin
-
-
-
?
2.3.1.81
acetyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-acetyl-kanamycin A
-
-
-
?
2.3.1.81
acetyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-acetyl-neomycin B
-
-
-
?
2.3.1.81
acetyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-acetyl-paromomycin
-
-
-
?
2.3.1.81
acetyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-acetyl-sisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-acetyl-tobramycin
-
-
-
?
2.3.1.81
butyryl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-butyryl-kanamycin A
-
-
-
?
2.3.1.81
glutaryl-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
glycyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-glycyl-amikacin
-
-
-
?
2.3.1.81
glycyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-glycyl-gentamicin
-
-
-
?
2.3.1.81
glycyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-glycyl-kanamycin A
-
-
-
?
2.3.1.81
glycyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-glycylneomycin B
-
-
-
?
2.3.1.81
glycyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-glycyl-sisomicin
-
-
-
?
2.3.1.81
malonyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-malonyl-amikacin
-
-
-
?
2.3.1.81
malonyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-malonyl-gentamicin
-
-
-
?
2.3.1.81
malonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-malonylneomycin B
-
-
-
?
2.3.1.81
methylmalonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-methylmalonylneomycin B
-
-
-
?
2.3.1.81
additional information
no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'')
703205
Escherichia coli
?
-
-
-
-
2.3.1.81
N-propionyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-propionyl-amikacin
-
-
-
?
2.3.1.81
N-propionyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-propionyl-gentamicin
-
-
-
?
2.3.1.81
N-propionyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-propionyl-kanamycin A
-
-
-
?
2.3.1.81
N-propionyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-propionylneomycin B
-
-
-
?
2.3.1.81
N-propionyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-propionyl-paromomycin
-
-
-
?
2.3.1.81
N-propionyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-propionyl-sisomicin
-
-
-
?
2.3.1.81
N-propionyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-propionyl-tobramycin
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.81
0.012
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.017
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.041
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.044
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.076
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.089
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.121
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.123
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.124
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.126
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.134
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.144
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.146
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.163
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.173
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.179
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.182
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.188
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.3
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.559
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.81
6.6
-
assay at
Escherichia coli
Application (protein specific)
EC Number
Application
Commentary
Organism
2.3.1.81
synthesis
development of a methodology that utilizes aminoglycoside acetyltransferase and unnatural acyl-CoA analogues for the chemoenzymatic generation of regioselectively N-acylatedaminoglycoside analogues. Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.81
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.81
0.00057
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.00096
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0015
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.002
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.0021
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0027
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0029
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0031
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0033
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0046
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0048
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.0054
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0055
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0091
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0099
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0111
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0135
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.0148
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0189
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.0716
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + amikacin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + gentamicin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + paromomycin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
4-bromo-thiophene-2-carbonyl-S-CoA + tobramycin
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
5-bromo-thiophene-2-carbonyl-S-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
5-bromo-thiophene-2-carbonyl-S-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
6-fluoropicolinyl-CoA + neomycin B
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
acetyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-acetyl-amikacin
-
-
-
?
2.3.1.81
acetyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-acetyl-gentamicin
-
-
-
?
2.3.1.81
acetyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-acetyl-kanamycin A
-
-
-
?
2.3.1.81
acetyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-acetyl-neomycin B
-
-
-
?
2.3.1.81
acetyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-acetyl-paromomycin
-
-
-
?
2.3.1.81
acetyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-acetyl-sisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-acetyl-tobramycin
-
-
-
?
2.3.1.81
butyryl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-butyryl-kanamycin A
-
-
-
?
2.3.1.81
glutaryl-CoA + kanamycin A
-
703205
Escherichia coli
?
-
-
-
?
2.3.1.81
glycyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-glycyl-amikacin
-
-
-
?
2.3.1.81
glycyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-glycyl-gentamicin
-
-
-
?
2.3.1.81
glycyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-glycyl-kanamycin A
-
-
-
?
2.3.1.81
glycyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-glycylneomycin B
-
-
-
?
2.3.1.81
glycyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-glycyl-sisomicin
-
-
-
?
2.3.1.81
malonyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-malonyl-amikacin
-
-
-
?
2.3.1.81
malonyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-malonyl-gentamicin
-
-
-
?
2.3.1.81
malonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-malonylneomycin B
-
-
-
?
2.3.1.81
methylmalonyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-methylmalonylneomycin B
-
-
-
?
2.3.1.81
additional information
no activity with benzoyl-CoA, acetoacetyl-CoA, crotonyl-CoA, isovaleryl-CoA, palmitoyl-CoA, and DL-3-hydroxy-butyryl-CoA by AAC(6')-APH(2'')
703205
Escherichia coli
?
-
-
-
-
2.3.1.81
N-propionyl-CoA + amikacin
-
703205
Escherichia coli
CoA + N3'-propionyl-amikacin
-
-
-
?
2.3.1.81
N-propionyl-CoA + gentamicin
-
703205
Escherichia coli
CoA + N3'-propionyl-gentamicin
-
-
-
?
2.3.1.81
N-propionyl-CoA + kanamycin A
-
703205
Escherichia coli
CoA + N3'-propionyl-kanamycin A
-
-
-
?
2.3.1.81
N-propionyl-CoA + neomycin B
-
703205
Escherichia coli
CoA + N3-propionylneomycin B
-
-
-
?
2.3.1.81
N-propionyl-CoA + paromomycin
-
703205
Escherichia coli
CoA + N3'-propionyl-paromomycin
-
-
-
?
2.3.1.81
N-propionyl-CoA + sisomicin
-
703205
Escherichia coli
CoA + N3'-propionyl-sisomicin
-
-
-
?
2.3.1.81
N-propionyl-CoA + tobramycin
-
703205
Escherichia coli
CoA + N3'-propionyl-tobramycin
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.81
0.012
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.017
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a
Escherichia coli
2.3.1.81
0.041
-
gentamicin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.044
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.076
-
neomycin B
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.089
-
tobramycin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.121
-
sisomicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.123
-
gentamicin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.124
-
amikacin
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.126
-
amikacin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.134
-
kanamycin A
pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.144
-
kanamycin A
pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.146
-
amikacin
pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.163
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
2.3.1.81
0.173
-
sisomicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.179
-
tobramycin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.182
-
gentamicin
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.188
-
neomycin B
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.3
-
kanamycin A
pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b
Escherichia coli
2.3.1.81
0.559
-
kanamycin A
pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.81
6.6
-
assay at
Escherichia coli