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Literature summary extracted from

  • Ruldeekulthamrong, P.; Maeda, S.; Kato, S.; Shinji, N.; Sittipraneed, S.; Packdibamrung, K.; Misono, H.
    Molecular characterization of lysine 6-dehydrogenase from Achromobacter denitrificans (2008), BMB Rep., 41, 790-795.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.18 cloned and overexpressed in Escherichia coli Achromobacter denitrificans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.18 using the hanging drop method Achromobacter denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.18 6-amino-n-caproate inhibition 20% by 10 mM 6-amino-n-caproate Achromobacter denitrificans
1.4.1.18 HgCl2
-
 Achromobacter denitrificans
1.4.1.18 L-leucine inhibition 50% by 10 mM L-leucine Achromobacter denitrificans
1.4.1.18 L-lysine
-
 Achromobacter denitrificans
1.4.1.18 L-norleucine inhibition 20% by 10 mM L-norleucine Achromobacter denitrificans
1.4.1.18 L-ornithine inhibition 10% by 10 mM L-ornithine Achromobacter denitrificans
1.4.1.18 N-ethylmaleimide
-
 Achromobacter denitrificans
1.4.1.18 p-chloromercuribenzoate
-
 Achromobacter denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.18 0.09
-
 NAD+
-
 Achromobacter denitrificans
1.4.1.18 0.11
-
 deamino-NAD+
-
 Achromobacter denitrificans
1.4.1.18 0.37
-
 nicotinamide guanine dinucleotide
-
 Achromobacter denitrificans
1.4.1.18 1.32
-
 3-acetylpyridine-NAD+
-
 Achromobacter denitrificans
1.4.1.18 5
-
 L-lysine
-
 Achromobacter denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.18 39360
-
 predicted from cDNA Achromobacter denitrificans
1.4.1.18 40000
-
 SDS-PAGE Achromobacter denitrificans
1.4.1.18 80000
-
 gel filtration, homodimer Achromobacter denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.18 Achromobacter denitrificans Q3S559
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.18 enzyme is purified approximately 100fold to homogeneity with a 15% yield Achromobacter denitrificans

Storage Stability

EC Number Storage Stability Organism
1.4.1.18 4°C, full activity is maintained for 2 months Achromobacter denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.18 L-lysine + 3-acetylpyridine-NAD+ + H2O
-
 Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + 3-acetylpyridine-NADH
-
 ?
1.4.1.18 L-lysine + deamino-NAD+ + H2O
-
 Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + deamino-NADH
-
 ?
1.4.1.18 L-lysine + NAD+ + H2O
-
 Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + NADH
-
 ?
1.4.1.18 L-lysine + nicotinamide guanine dinucleotide + H2O
-
 Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + nicotinamide guanine dinucleotide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.18 hexamer 6 * 40000 Da, gel filtration, enzyme elutes as a hexamer when a high concentration of L-lysine (10 mM) is supplemented to both the enzyme and the elution buffer Achromobacter denitrificans
1.4.1.18 homodimer 2 * 40000 Da, gel filtration Achromobacter denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.4.1.18 Lys 6-DH
-
 Achromobacter denitrificans
1.4.1.18 lysine 6-dehydrogenase
-
 Achromobacter denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.18 50
-
-
 Achromobacter denitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.18 30
-
 at 30°C enzyme activity is kept for 3 days and 70% of the activity remains after 7 days Achromobacter denitrificans
1.4.1.18 50
-
 enzyme is stable up to 50°C when heated for 10 min in KP buffer (pH 7.4) Achromobacter denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.18 9.7
-
 optimal pH Achromobacter denitrificans
1.4.1.18 10
-
 assay at Achromobacter denitrificans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.18 5.5 7
-
 Achromobacter denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.18 3-acetylpyridine-NAD+
-
 Achromobacter denitrificans
1.4.1.18 deamino-NAD+
-
 Achromobacter denitrificans
1.4.1.18 NAD+ no activity with NADP+ Achromobacter denitrificans
1.4.1.18 nicotinamide guanine dinucleotide
-
 Achromobacter denitrificans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.18 3.2
-
 L-lysine
-
 Achromobacter denitrificans