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Literature summary extracted from

  • Kim, D.; Ortiz de Montellano, P.R.
    Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system (2009), Biotechnol. Lett., 31, 1427-1431.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.15.1 biotechnology bioengineered Escherichia coli cells possess a heterologous self-sufficient P450 catalytic system that may have advantages in terms of low cost and high yield for the production of fine chemicals Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.161 expression in Escherichia coli Pseudomonas putida
1.14.15.1 functional expression in Escherichia coli DH5alpha of tricistronic constructs consisting of P450cam encoded by the first cistron and the auxiliary proteins, putidaredoxin and putidaredoxin reductase by the second and the third Pseudomonas putida
1.18.1.5 expression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.161 additional information functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system Pseudomonas putida
1.18.1.5 additional information functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 5-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.1 47000
-
 x * 47000, SDS-PAGE/Coomassie staining Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.161 Pseudomonas putida
-
-
-
1.14.15.1 Pseudomonas putida
-
-
-
1.18.1.5 Pseudomonas putida P16640
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.161 (+)-camphor + NADPH + H+ + O2
-
 Pseudomonas putida (+)-6-exo-hydroxycamphor + NADP+ + H2O
-
 ?
1.14.13.161 limonene + NADPH + H+ + O2
-
 Pseudomonas putida (-)-perillyl alcohol + NADP+ + H2O
-
 ?
1.14.15.1 (1R)-(+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 (4S)-limonene + putidaredoxin + O2 the 7-position is the major site of hydroxylation by P450cam Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.1 ? x * 47000, SDS-PAGE/Coomassie staining Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.161 P450cam
-
 Pseudomonas putida
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin
-
 Pseudomonas putida