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Literature summary extracted from

  • Feng, Y.; Duan, C.J.; Liu, L.; Tang, J.L.; Feng, J.X.
    Properties of a metagenome-derived beta-glucosidase from the contents of rabbit cecum (2009), Biosci. Biotechnol. Biochem., 73, 1470-1473.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 D772A the mutant shows less than 1/10000 activity of the wild type enzyme uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cr3+ 42% residual activity at 5 mM uncultured bacterium
3.2.1.21 Cu2+ 86% residual activity at 5 mM uncultured bacterium
3.2.1.21 EDTA effective inhibitor, 32% residual activity at 5 mM uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ 124% relative activity at 5 mM uncultured bacterium
3.2.1.21 Co2+ 129% relative activity at 5 mM uncultured bacterium
3.2.1.21 Fe2+ 107% relative activity at 5 mM uncultured bacterium
3.2.1.21 Mg2+ 106% relative activity at 5 mM uncultured bacterium
3.2.1.21 Mn2+ 108% relative activity at 5 mM uncultured bacterium
3.2.1.21 additional information not stimulated by Zn2+, Li+, Fe3+, and K+ uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 100000
-
 SDS-PAGE uncultured bacterium
3.2.1.21 101000
-
 calculated from amino acid sequence uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 uncultured bacterium Q3HXC2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 Ni-NTA column chromatography and Mono Q column chromatography uncultured bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2.91
-
 crude extract uncultured bacterium
3.2.1.21 46.3
-
 purified enzyme uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O low activity uncultured bacterium 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 uncultured bacterium 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity uncultured bacterium 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.21 cellobiose + H2O low activity uncultured bacterium 2 beta-D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O low activity uncultured bacterium 4 beta-D-glucose
-
 ?
3.2.1.21 cellotriose + H2O low activity uncultured bacterium 3 D-glucose
-
 ?
3.2.1.21 additional information no hydrolysis of xylan, cellopentaose, cellohexaose, and carboxymethylcellulose uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Umbgl3B
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
 uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 10 50 the enzyme is stable from 10-30°C, about 75% activity at 40°C, about 30% activity at 50°C, almost inactive at 50°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 3 10 about 60% activity at pH 3.0, about 80% activity at pH 4.0, the enzyme is stable at pH 5.0-9.0, activity drops sharply at pH 10.0 uncultured bacterium