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Literature summary extracted from

  • Pal, S.; Banik, S.P.; Ghorai, S.; Chowdhury, S.; Khowala, S.
    Purification and characterization of a thermostable intra-cellular beta-glucosidase with transglycosylation properties from filamentous fungus Termitomyces clypeatus (2010), Biores. Technol., 101, 2412-2420.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 beta-mercaptoethanol the purified enzyme shows 152.9% relative activity at 1% (v/v) Termitomyces clypeatus
3.2.1.21 dithiothreitol the purified enzyme shows 108.4% relative activity at 20 mM Termitomyces clypeatus

Application

EC Number Application Comment Organism
3.2.1.21 synthesis the enzyme is useful in synthetic biology to produce complex bioactive glycosides and to avoid chemical hazards Termitomyces clypeatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2-deoxy-D-glucose
-
 Termitomyces clypeatus
3.2.1.21 beta-mercaptoethanol the mycelial extract shows 87.1% residual activity at 1% (v/v) Termitomyces clypeatus
3.2.1.21 Ca2+ the mycelial extract shows 59.5% residual activity at 20 mM, the purified enzyme shows 53.5% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 Cu2+ the mycelial extract shows 58.1% residual activity at 2 mM, the purified enzyme shows 44.4% residual activity at 2 mM Termitomyces clypeatus
3.2.1.21 dithiothreitol the mycelial extract shows 75.7% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 EDTA the mycelial extract shows 64.3% residual activity at 20 mM, the purified enzyme shows 37.4% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 Hg2+ the mycelial extract shows 26.8% residual activity at 20 mM, the purified enzyme shows 1.2% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 K+ the mycelial extract shows 70.9% residual activity at 20 mM, the purified enzyme shows 41.5% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 Mg2+ the mycelial extract shows 62.1% residual activity at 20 mM, the purified enzyme shows 39.2% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 Mn2+ the mycelial extract shows 42.9% residual activity at 20 mM, the purified enzyme shows 26.3% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 p-chloromercuribenzoic acid the mycelial extract shows 66.9% residual activity at 0.5 mM, the purified enzyme shows 36.9% residual activity at 0.5 mM Termitomyces clypeatus
3.2.1.21 SDS the mycelial extract shows no residual activity at 0.05% (v/v), purified enzyme shows 50% residual activity at 0.05% (v/v) Termitomyces clypeatus
3.2.1.21 Sodium azide the mycelial extract shows 67.6% residual activity at 20 mM, the purified enzyme shows 42.7% residual activity at 20 mM Termitomyces clypeatus
3.2.1.21 Triton X-100 the mycelial extract shows 70.6% residual activity at 1% (v/v), the purified enzyme is not inhibited by 1% (v/v) Triton X-100 Termitomyces clypeatus
3.2.1.21 Urea the mycelial extract shows 8% residual activity at 4 M, the purified enzyme shows 66% residual activity at 4 M Termitomyces clypeatus
3.2.1.21 Zn2+ the mycelial extract shows 38.6% residual activity at 20 mM, the purified enzyme shows 30.3% residual activity at 20 mM Termitomyces clypeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.131
-
 4-nitrophenyl-beta-D-glucopyranoside mycelial extract Termitomyces clypeatus
3.2.1.21 0.148
-
 4-nitrophenyl-beta-D-glucopyranoside purified enzyme Termitomyces clypeatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 6688
-
 MALDI-TOF mass spectrometry, in the presence of 2-deoxy-D-glucose Termitomyces clypeatus
3.2.1.21 116000
-
 SDS-PAGE Termitomyces clypeatus
3.2.1.21 125900
-
 HPGPLC column chromatography Termitomyces clypeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Termitomyces clypeatus
-
-
-
3.2.1.21 Termitomyces clypeatus MTCC 5091
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 HPGPLC column chromatography, Sephacryl S-200 gel filtration and DEAE Sephadex gel filtration Termitomyces clypeatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 mycelium
-
 Termitomyces clypeatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.09
-
 purified enzyme, using methyl beta-D-glucopyranoside as substrate Termitomyces clypeatus
3.2.1.21 0.14
-
 purified enzyme, using salicin as substrate Termitomyces clypeatus
3.2.1.21 0.15
-
 purified enzyme, using cellobiose as substrate Termitomyces clypeatus
3.2.1.21 0.54
-
 purified enzyme, using 4-nitrophenyl.beta-D-glucopyranoside as substrate Termitomyces clypeatus
3.2.1.21 43.3
-
 mycelial extract Termitomyces clypeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O the enzyme had higher specific activity against 4-nitrophenyl beta-D-glucopyranoside than cellobiose Termitomyces clypeatus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O the enzyme had higher specific activity against 4-nitrophenyl beta-D-glucopyranoside than cellobiose Termitomyces clypeatus MTCC 5091 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Termitomyces clypeatus 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Termitomyces clypeatus MTCC 5091 2 beta-D-glucose
-
 ?
3.2.1.21 methyl beta-D-glucopyranoside + H2O
-
 Termitomyces clypeatus ?
-
 ?
3.2.1.21 methyl beta-D-glucopyranoside + H2O
-
 Termitomyces clypeatus MTCC 5091 ?
-
 ?
3.2.1.21 additional information the purified enzyme shows substrate specificity only towards substrates with beta-1,4 linkages and displays no hydrolytic activity towards 2-nitrophenyl apha-D-galactopyranoside, methyl alpha-D-galactopyranoside, melibiose, carboxymethyl cellulose, and starch Termitomyces clypeatus ?
-
 ?
3.2.1.21 additional information the purified enzyme shows substrate specificity only towards substrates with beta-1,4 linkages and displays no hydrolytic activity towards 2-nitrophenyl apha-D-galactopyranoside, methyl alpha-D-galactopyranoside, melibiose, carboxymethyl cellulose, and starch Termitomyces clypeatus MTCC 5091 ?
-
 ?
3.2.1.21 salicin + H2O
-
 Termitomyces clypeatus ?
-
 ?
3.2.1.21 salicin + H2O
-
 Termitomyces clypeatus MTCC 5091 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 cellobiase
-
 Termitomyces clypeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 45
-
-
 Termitomyces clypeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40 80 the purified enzyme has about 80% and 46% residual activities at 60°C and 80°C, unpurified mycelia extract enzyme has 86%, 60% and 22% residual activities at 40°C, 60°C, and 80°C, respectively. The thermal stability 70°C is 64% Termitomyces clypeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5 9 residual activity is 80-100% between pH ranges 5.0-8.0, the stability of the enzyme in mycelia extract is comparatively low at acidic pH and the enzyme is most stable at pH 9.0, the purified enzyme shows more than 95% residual activity between pH ranges 6.0-7.0 Termitomyces clypeatus