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Literature summary extracted from

  • Letelier, M.E.; Molina-Berrios, A.; Cortes-Troncoso, J.; Jara-Sandoval, J.A.; Mueller, A.; Aracena-Parks, P.
    Comparative effects of superoxide anion and hydrogen peroxide on microsomal and cytosolic glutathione S-transferase activities of rat liver (2010), Biol. Trace Elem. Res., 134, 203-211.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.18 H2O2 inhibits cytosolic GSTs and activates microsomal GSTs Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 H2O2 inhibits cytosolic GST by about 65% and activates microsomal GST to about 175% Rattus norvegicus
2.5.1.18 additional information presence of superoxide dismutase and catalase reduces the activity of the microsomal GST isozyme, but only catalase can reduce the activity of the cytosolic GST Rattus norvegicus
2.5.1.18 superoxide anion generated by a Fe3+/ascorbate system, inhibits cytosolic GSTs and microsomal GSTs by 30% and 65%, respectively. Addition of 2 mM DTT fails to completely reverse microsomal GST inhibition elicited by Fe3+/ascorbate, recovering only about 10% of this activity, but it completely reverses the inhibitory effect of Fe3+/ascorbate on cytosolic GST activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Rattus norvegicus 5829
-
2.5.1.18 membrane integral Rattus norvegicus 16020
-
2.5.1.18 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Rattus norvegicus
-
 male sprague-dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.05
-
 microsomal isozyme in presence of catalase Rattus norvegicus
2.5.1.18 0.06
-
 microsomal isozyme in presence of superoxide dismutase Rattus norvegicus
2.5.1.18 0.075
-
 microsomal isozyme in presence of Fe3+/ascorbate Rattus norvegicus
2.5.1.18 0.139
-
 microsomal isozyme Rattus norvegicus
2.5.1.18 0.692
-
 cytosolic isozyme in presence of Fe3+/ascorbate Rattus norvegicus
2.5.1.18 0.702
-
 cytosolic isozyme in presence of catalase Rattus norvegicus
2.5.1.18 0.984
-
 cytosolic isozyme in presence of superoxide dismutase Rattus norvegicus
2.5.1.18 0.985
-
 cytosolic isozyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Rattus norvegicus
2.5.1.18 GST
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 37
-
 assay at Rattus norvegicus