EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.68 | expressed in Escherichia coli BL21 cells and in Nicotiana tabacum cultivar Wisconsin 38 | Triticum aestivum |
2.1.1.104 | DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Triticum aestivum |
2.1.1.104 | recombinant expression in transgenic plants using the transformation by Agrobacterium tumefaciens strain LBA4404 | Nicotiana tabacum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.104 | additional information | construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview | Triticum aestivum |
2.1.1.104 | additional information | construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum, phenotype, overview | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.68 | 0.01731 | - |
5-hydroxyconiferaldehyde | - |
Triticum aestivum | |
2.1.1.68 | 0.04372 | - |
caffeoyl aldehyde | - |
Triticum aestivum | |
2.1.1.68 | 0.06875 | - |
3,4-dihydroxy-trans-cinnamate | - |
Triticum aestivum | |
2.1.1.68 | 0.08403 | - |
caffeoyl alcohol | - |
Triticum aestivum | |
2.1.1.68 | 0.1002 | - |
5-hydroxyconiferyl alcohol | - |
Triticum aestivum | |
2.1.1.104 | 0.0173 | - |
5-hydroxy-coniferaldehyde | - |
Triticum aestivum | |
2.1.1.104 | 0.0437 | - |
caffeoyl aldehyde | - |
Triticum aestivum | |
2.1.1.104 | 0.0688 | - |
caffeic acid | - |
Triticum aestivum | |
2.1.1.104 | 0.083 | - |
caffeoyl-CoA | - |
Triticum aestivum | |
2.1.1.104 | 0.0952 | - |
5-hydroxy-feruloyl-CoA | - |
Triticum aestivum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.68 | 40000 | - |
SDS-PAGE | Triticum aestivum |
2.1.1.104 | 38600 | - |
x * 38600, about, sequence calculation | Triticum aestivum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.104 | additional information | Triticum aestivum | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | ? | - |
? | |
2.1.1.104 | additional information | Nicotiana tabacum | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | ? | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + caffeoyl-CoA | Triticum aestivum | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.68 | Triticum aestivum | Q38J50 | cultivar H4564 | - |
2.1.1.104 | Nicotiana tabacum | O24149 | cv. Wisconsin 38 | - |
2.1.1.104 | Triticum aestivum | Q38J50 | cv. H4564 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.68 | Ni-NTA column chromatography | Triticum aestivum |
2.1.1.104 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants | Triticum aestivum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.68 | leaf | - |
Triticum aestivum | - |
2.1.1.68 | root | - |
Triticum aestivum | - |
2.1.1.68 | stem | - |
Triticum aestivum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.104 | additional information | - |
determination of lignin content in transgenic plants, overview | Nicotiana tabacum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.68 | additional information | the enzyme exhibits also a strong activity towards ester precursors including caffeoyl-CoA and 5-hydroxyferuloyl-CoA | Triticum aestivum | ? | - |
? | |
2.1.1.68 | S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Triticum aestivum | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
2.1.1.68 | S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | exhibits the highest catalyzing efficiency towards 5-hydroxyconiferaldehyde | Triticum aestivum | S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde | - |
? | |
2.1.1.68 | S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol | - |
Triticum aestivum | S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol | - |
? | |
2.1.1.68 | S-adenosyl-L-methionine + caffeoyl alcohol | - |
Triticum aestivum | S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol | - |
? | |
2.1.1.68 | S-adenosyl-L-methionine + caffeoyl aldehyde | exhibits the highest catalyzing efficiency towards caffeoyl aldehyde | Triticum aestivum | S-adenosyl-L-homocysteine + coniferyl aldehyde | - |
? | |
2.1.1.104 | additional information | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | Triticum aestivum | ? | - |
? | |
2.1.1.104 | additional information | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | Nicotiana tabacum | ? | - |
? | |
2.1.1.104 | additional information | the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview | Triticum aestivum | ? | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Triticum aestivum | S-adenosyl-L-homocysteine + sinapaldehyde | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + caffeic acid | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ferulic acid | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + caffeoyl alcohol | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.104 | S-adenosyl-L-methionine + caffeoyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.104 | ? | x * 38600, about, sequence calculation | Triticum aestivum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.68 | caffeic acid 3-O-methyltransferase | - |
Triticum aestivum |
2.1.1.68 | COMT | - |
Triticum aestivum |
2.1.1.104 | caffeic acid 3-O-methyltransferase | - |
Triticum aestivum |
2.1.1.104 | caffeic acid 3-O-methyltransferase | - |
Nicotiana tabacum |
2.1.1.104 | COMT | - |
Triticum aestivum |
2.1.1.104 | COMT | - |
Nicotiana tabacum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.104 | S-adenosyl-L-methionine | - |
Triticum aestivum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.104 | metabolism | COMT plays a pivotal position in the lignin biosynthetic pathway | Triticum aestivum |
2.1.1.104 | metabolism | COMT plays a pivotal position in the lignin biosynthetic pathway | Nicotiana tabacum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.68 | 0.0011 | - |
5-hydroxyconiferyl alcohol | - |
Triticum aestivum | |
2.1.1.68 | 0.0014 | - |
3,4-dihydroxy-trans-cinnamate | - |
Triticum aestivum | |
2.1.1.68 | 0.0017 | - |
caffeoyl alcohol | - |
Triticum aestivum | |
2.1.1.68 | 0.0024 | - |
caffeoyl aldehyde | - |
Triticum aestivum | |
2.1.1.68 | 0.0037 | - |
5-hydroxyconiferaldehyde | - |
Triticum aestivum |