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Literature summary extracted from

  • Van Vleet, J.; Kleeb, A.; Kast, P.; Hilvert, D.; Cleland, W.W.
    13C isotope effect on the reaction catalyzed by prephenate dehydratase (2010), Biochim. Biophys. Acta, 1804, 752-754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.51 overexpressed in Escherichia coli KA13, transformed with the plasmid pAKZ11 Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Methanocaldococcus jannaschii
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.51
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Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate 13C isotope effect for conversion of prephenate is 1.0334, the size of this isotope effect suggests that the reaction is concerted. Only residue capable of acting as the general acid needed for removal of the hydroxyl group is threonine-172, which is contained in a conserved TRF motif. More favorable entropy of activation for the enzyme-catalyzed process (25 eu larger than for the acid-catalyzed reaction) may be due to preorganized microenvironment that obviates the need for extensive solvent reorganization, which is consistent with forced planarity of the ring and side chain, which may place the leaving carboxyl and hydroxyl out of plane. Such distortion of the substrate may be a major contributor to catalysis Methanocaldococcus jannaschii phenylpyruvate + H2O + CO2
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Synonyms

EC Number Synonyms Comment Organism
4.2.1.51 PDT
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Methanocaldococcus jannaschii
4.2.1.51 prephenate dehydratase
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Methanocaldococcus jannaschii