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Literature summary extracted from

  • Meng, M.; Fitzek, E.; Gajowniczek, A.; Wilczynska, M.; Kleczkowski, L.A.
    Domain-specific determinants of catalysis/substrate binding and the oligomerization status of barley UDP-glucose pyrophosphorylase (2009), Biochim. Biophys. Acta, 1794, 1734-1742.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 His-tag version expressed in Escherichia coli BL21 (DE3) Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.9 Ccut-101 101 amino acid residues deleted (exons 16-19, and 1 amino acid residue of exon 15) Hordeum vulgare
2.7.7.9 Ccut-32 32 amino acid residues deleted (exon 18 and exon 19) Hordeum vulgare
2.7.7.9 Ccut-67 67 amino acid residues deleted (exons 17-19) Hordeum vulgare
2.7.7.9 Ccut-8 8 amino acid residues deleted (last exon (exon 19)) Hordeum vulgare
2.7.7.9 Delta 1-4 deletion of 4 amino acid residues of the C-terminal domain, possibly involved in oligomerization Hordeum vulgare
2.7.7.9 Delta 1-8 deletion of 8 amino acid residues of the C-terminal domain, possibly involved in oligomerization Hordeum vulgare
2.7.7.9 Delta-NB deletion mutant: amino acid residue 96-100 deleted (essential for catalysis) Hordeum vulgare
2.7.7.9 K183A possibly involved in subunit interaction Hordeum vulgare
2.7.7.9 K260A possibly involved in diphosphate binding Hordeum vulgare
2.7.7.9 K332A possibly involved in subunit interaction Hordeum vulgare
2.7.7.9 K405A possibly involved in subunit interaction Hordeum vulgare
2.7.7.9 Ncut-21 21 amino acid residues deleted (exon 1) Hordeum vulgare
2.7.7.9 Ncut-37 37 amino acid residues deleted (exon 1 and exon 2) Hordeum vulgare
2.7.7.9 Y192A possibly involved in UDP-glucose binding Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.027
-
 diphosphate Y192A mutant protein Hordeum vulgare
2.7.7.9 0.03
-
 diphosphate Ccut-8 mutant protein Hordeum vulgare
2.7.7.9 0.03
-
 UDP-glucose Ccut-8 mutant protein Hordeum vulgare
2.7.7.9 0.03
-
 diphosphate K183A mutant protein Hordeum vulgare
2.7.7.9 0.03
-
 UDP-glucose K183A mutant protein Hordeum vulgare
2.7.7.9 0.03
-
 UDP-glucose K332A mutant protein Hordeum vulgare
2.7.7.9 0.031
-
 UDP-glucose Y192A mutant protein Hordeum vulgare
2.7.7.9 0.034
-
 UDP-glucose wild-type protein Hordeum vulgare
2.7.7.9 0.039
-
 diphosphate wild-type protein Hordeum vulgare
2.7.7.9 0.04
-
 diphosphate K332A mutant protein Hordeum vulgare
2.7.7.9 0.04
-
 UDP-glucose K405A mutant protein Hordeum vulgare
2.7.7.9 0.074
-
 UDP-glucose K260A mutant protein Hordeum vulgare
2.7.7.9 0.09
-
 diphosphate Ncut-21 mutant protein Hordeum vulgare
2.7.7.9 0.118
-
 UDP-glucose Ccut-101 mutant protein Hordeum vulgare
2.7.7.9 0.24
-
 diphosphate K405A mutant protein Hordeum vulgare
2.7.7.9 0.31
-
 UDP-glucose Ccut-32 mutant protein Hordeum vulgare
2.7.7.9 0.362
-
 UDP-glucose Ccut-67 mutant protein Hordeum vulgare
2.7.7.9 0.503
-
 UDP-glucose Delta 1-8 mutant protein Hordeum vulgare
2.7.7.9 0.555
-
 UDP-glucose Delta 1-4 mutant protein Hordeum vulgare
2.7.7.9 0.9
-
 diphosphate Ncut-37 mutant protein Hordeum vulgare
2.7.7.9 1.1
-
 UDP-glucose Ncut-21 mutant protein Hordeum vulgare
2.7.7.9 4.9
-
 diphosphate K260A mutant protein Hordeum vulgare
2.7.7.9 6.4
-
 diphosphate Ccut-32 mutant protein Hordeum vulgare
2.7.7.9 9.8
-
 diphosphate Ccut-67 mutant protein Hordeum vulgare
2.7.7.9 29.4
-
 diphosphate Delta 1-4 mutant protein Hordeum vulgare
2.7.7.9 33.3
-
 diphosphate Ccut-101 mutant protein Hordeum vulgare
2.7.7.9 57
-
 UDP-glucose Ncut-37 mutant protein Hordeum vulgare
2.7.7.9 60
-
 diphosphate Delta 1-8 mutant protein Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Hordeum vulgare Q43772
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 immobilized metal ion affinity chromatography Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9
-
-
 Delta-NB mutant protein Hordeum vulgare
2.7.7.9 0.02
-
 Ncut-37 mutant protein Hordeum vulgare
2.7.7.9 0.21
-
 Ccut-101 mutant protein Hordeum vulgare
2.7.7.9 0.27
-
 Ccut-32 mutant protein Hordeum vulgare
2.7.7.9 0.42
-
 K260A mutant protein Hordeum vulgare
2.7.7.9 2.1
-
 Delta 1-4 mutant protein Hordeum vulgare
2.7.7.9 3.1
-
 Ccut-67 mutant protein Hordeum vulgare
2.7.7.9 5.4
-
 Delta 1-8 mutant protein Hordeum vulgare
2.7.7.9 12.8
-
 Ncut-21 mutant protein Hordeum vulgare
2.7.7.9 591
-
 Y192A mutant protein Hordeum vulgare
2.7.7.9 752
-
 K183A mutant protein Hordeum vulgare
2.7.7.9 764
-
 K405A mutant protein Hordeum vulgare
2.7.7.9 853
-
 K332A mutant protein Hordeum vulgare
2.7.7.9 1074
-
 wild-type protein Hordeum vulgare
2.7.7.9 1547
-
 Ccut-8 mutant protein Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + UDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.9 oligomer x * 52000-53000, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UDP-glucose pyrophosphorylase
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.9 Hordeum vulgare K183A mutant protein
-
 5.47
2.7.7.9 Hordeum vulgare K332A mutant protein
-
 5.47
2.7.7.9 Hordeum vulgare K405A mutant protein
-
 5.47
2.7.7.9 Hordeum vulgare Ncut-21 mutant protein
-
 5.54
2.7.7.9 Hordeum vulgare Delta 1-8 mutant protein
-
 5.55
2.7.7.9 Hordeum vulgare Delta-NB mutant protein
-
 5.55
2.7.7.9 Hordeum vulgare wild-type protein
-
 5.55
2.7.7.9 Hordeum vulgare Y192A mutant protein
-
 5.55
2.7.7.9 Hordeum vulgare Ccut-101 mutant protein
-
 5.58
2.7.7.9 Hordeum vulgare Delta 1-4 mutant protein
-
 5.63
2.7.7.9 Hordeum vulgare Ccut-67 mutant protein
-
 5.69
2.7.7.9 Hordeum vulgare Ncut-37 mutant protein
-
 5.7
2.7.7.9 Hordeum vulgare Ccut-32 mutant protein
-
 5.79
2.7.7.9 Hordeum vulgare Ccut-8 mutant protein
-
 5.8