BRENDA - Enzyme Database show

Characterisation of dihydrodipicolinate synthase (DHDPS) from Bacillus anthracis

Domigan, L.J.; Scally, S.W.; Fogg, M.J.; Hutton, C.A.; Perugini, M.A.; Dobson, R.C.; Muscroft-Taylor, A.C.; Gerrard, J.A.; Devenish, S.R.; Biochim. Biophys. Acta 1794, 1510-1516 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
L-methionine
at 10 mM 112% activity, at 100 mM 111% activity
Escherichia coli
4.3.3.7
meso-diaminopimelate
at 1 mM 108% activity, at 10 mM 122% activity
Escherichia coli
4.3.3.7
additional information
meso-diaminopimelate has no effect
Bacillus anthracis
Application
EC Number
Application
Commentary
Organism
4.3.3.7
drug development
DHDPS is a potential antibiotic target
Bacillus anthracis
Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
plasmid pB3935 containing a His-tagged copy of the dapA2 gene and a gene conferring kanamycin resistance, transformed into Escherichia coli BL21(DE3) for protein overexpression
Bacillus anthracis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-aspartate 4-semialdehyde
DHDPS is subject to substrate inhibition by L-aspartate 4-semialdehyde
Bacillus anthracis
4.3.3.7
L-lysine
at 1 mM 23% residual activity, at 100 mM 13% residual activity
Escherichia coli
4.3.3.7
L-methionine
at 10 or 100 mM 80% residual activity
Bacillus anthracis
4.3.3.7
L-threonine
at 100 mM 40% residual activity
Bacillus anthracis
4.3.3.7
L-threonine
at 100 mM 23% residual activity, at 100 mM 33% residual activity
Escherichia coli
4.3.3.7
additional information
is not inhibited by 1-100 mM L-lysine or L-isoleucine
Bacillus anthracis
4.3.3.7
additional information
1-100 mM L-isoleucine has no effect
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.18
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis
4.3.3.7
0.43
-
pyruvate
-
Bacillus anthracis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
32290
-
mass spectrometry
Bacillus anthracis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Bacillus anthracis
Q81WN7
-
-
4.3.3.7
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
to homogeneity
Bacillus anthracis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
active sites are similiar to Bacillus anthracis DHDPS
702448
Escherichia coli
dihydrodipicolinate + 2 H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
active sites are similiar to Escherichia coli DHDPS
702448
Bacillus anthracis
dihydrodipicolinate + 2 H2O
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.3.7
59.8
-
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart
Bacillus anthracis
4.3.3.7
61.3
-
is thermally stabilised by the first substrate, pyruvate
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.3.7
76
-
pyruvate
-
Bacillus anthracis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3.3.7
5.4
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
L-methionine
at 10 mM 112% activity, at 100 mM 111% activity
Escherichia coli
4.3.3.7
meso-diaminopimelate
at 1 mM 108% activity, at 10 mM 122% activity
Escherichia coli
4.3.3.7
additional information
meso-diaminopimelate has no effect
Bacillus anthracis
Application (protein specific)
EC Number
Application
Commentary
Organism
4.3.3.7
drug development
DHDPS is a potential antibiotic target
Bacillus anthracis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
plasmid pB3935 containing a His-tagged copy of the dapA2 gene and a gene conferring kanamycin resistance, transformed into Escherichia coli BL21(DE3) for protein overexpression
Bacillus anthracis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-aspartate 4-semialdehyde
DHDPS is subject to substrate inhibition by L-aspartate 4-semialdehyde
Bacillus anthracis
4.3.3.7
L-lysine
at 1 mM 23% residual activity, at 100 mM 13% residual activity
Escherichia coli
4.3.3.7
L-methionine
at 10 or 100 mM 80% residual activity
Bacillus anthracis
4.3.3.7
L-threonine
at 100 mM 40% residual activity
Bacillus anthracis
4.3.3.7
L-threonine
at 100 mM 23% residual activity, at 100 mM 33% residual activity
Escherichia coli
4.3.3.7
additional information
is not inhibited by 1-100 mM L-lysine or L-isoleucine
Bacillus anthracis
4.3.3.7
additional information
1-100 mM L-isoleucine has no effect
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3.3.7
5.4
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.18
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis
4.3.3.7
0.43
-
pyruvate
-
Bacillus anthracis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
32290
-
mass spectrometry
Bacillus anthracis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
to homogeneity
Bacillus anthracis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
active sites are similiar to Bacillus anthracis DHDPS
702448
Escherichia coli
dihydrodipicolinate + 2 H2O
-
-
-
?
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
active sites are similiar to Escherichia coli DHDPS
702448
Bacillus anthracis
dihydrodipicolinate + 2 H2O
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.3.7
59.8
-
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart
Bacillus anthracis
4.3.3.7
61.3
-
is thermally stabilised by the first substrate, pyruvate
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.3.7
76
-
pyruvate
-
Bacillus anthracis