Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Walther, M.; Roffeis, J.; Jansen, C.; Anton, M.; Ivanov, I.; Kuhn, H.
    Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms (2009), Biochim. Biophys. Acta, 1791, 827-835.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.33 additional information positional specificity of wild-type 15-LOX-2 does hardly depend on the pH of the reaction buffer. The pH sensitivity can be induced by introduction of a His at the active site Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Mus musculus
1.13.11.31 ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Danio rerio
1.13.11.33 ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Mus musculus
1.13.11.33 ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Oryctolagus cuniculus
1.13.11.33 LOX isoforms ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Homo sapiens
1.13.11.40 ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.33 D602Y 15-LOX-2 mutant shows a similar pH-profile as the wild-type enzyme Homo sapiens
1.13.11.33 D602Y/V603H a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values Homo sapiens
1.13.11.33 V603H a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values. 15-H(p)ETE is the major oxygenation product at pH 8 Homo sapiens
1.13.11.40 H604F 8-LOX mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product. Specific activity of 8-LOX mutant with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate continously declines when pH increases Mus musculus
1.13.11.40 Y603F 8-LOX mutant, is relative insensitive towards pH alterations in the near physiological range (pH 6-8), at strong alkaline conditions (pH more than 9) significant shares of 15S-H(p)ETE are formed Mus musculus
1.13.11.40 Y603F/H604F 8-LOX double mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 additional information physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms Mus musculus
1.13.11.33 additional information physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms Homo sapiens
1.13.11.33 additional information physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms Mus musculus
1.13.11.33 additional information pH alterations in the near physiological range impact the iron content of LOX and thus the catalytic activity Oryctolagus cuniculus
1.13.11.40 additional information physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Fe
-
Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Danio rerio
-
-
-
1.13.11.31 Mus musculus
-
-
-
1.13.11.33 Homo sapiens
-
-
-
1.13.11.33 Mus musculus
-
-
-
1.13.11.33 Oryctolagus cuniculus
-
-
-
1.13.11.40 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 on Ni column Mus musculus
1.13.11.31 on Ni column Danio rerio
1.13.11.33 on Ni column Mus musculus
1.13.11.33 on Ni column Homo sapiens
1.13.11.33 on Ni column Oryctolagus cuniculus
1.13.11.40 on Ni column Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 skin
-
Mus musculus
-
1.13.11.33 skin
-
Mus musculus
-
1.13.11.40 skin
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.40 additional information
-
specific activity of 8-LOX with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate strongly increases going from pH 6 to 7.2 but then drops down at more alkaline pH Mus musculus

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -80°C, 10% glycerol Mus musculus
1.13.11.31 -80°C, 10% glycerol Danio rerio
1.13.11.33 -80°C, 10% glycerol Mus musculus
1.13.11.33 -80°C, 10% glycerol Homo sapiens
1.13.11.33 -80°C, 10% glycerol Oryctolagus cuniculus
1.13.11.40 -80°C, 10% glycerol Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate 12-H(p)ETE is the major reaction product of 12/15-LOX independent of the pH ?
1.13.11.31 arachidonate + O2
-
Danio rerio (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate 15-H(p)ETE is the major reaction product independent of the pH ?
1.13.11.33 arachidonate + O2
-
Mus musculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
1.13.11.33 arachidonate + O2
-
Oryctolagus cuniculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate 15-H(p)ETE is the major reaction product independent of the pH ?
1.13.11.33 arachidonate + O2
-
Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate 15-H(p)ETE is the major reaction product of 15-LOX-2 and 12/15-LOX independent of the pH ?
1.13.11.33 additional information 15-LOX-2 does not oxygenate (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid at various pH Homo sapiens ?
-
?
1.13.11.40 (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid + O2 8-LOX tolerates a carboxylic group in the substrate-binding pocket Mus musculus ?
-
?
1.13.11.40 arachidonate + O2
-
Mus musculus (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate 8-H(p)ETE is the major reaction product of 8-LOX independent of the pH ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-lipoxygenase
-
Mus musculus
1.13.11.31 12-LOX
-
Danio rerio
1.13.11.31 12/15-LOX
-
Mus musculus
1.13.11.33 12/15-LOX
-
Mus musculus
1.13.11.33 12/15-LOX
-
Homo sapiens
1.13.11.33 12/15-LOX
-
Oryctolagus cuniculus
1.13.11.33 15-lipoxygenase
-
Mus musculus
1.13.11.33 15-lipoxygenase-2
-
Homo sapiens
1.13.11.33 15-LOX-1
-
Homo sapiens
1.13.11.33 15-LOX-2
-
Homo sapiens
1.13.11.40 8-lipoxygenase
-
Mus musculus
1.13.11.40 8-LOX
-
Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.33 8 9 pH-optimum of 15-LOX-2 for arachidonic acid oxygenation Homo sapiens
1.13.11.40 8
-
8-LOX Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.33 6 9.5 pH 6 about 50% of maximal activity, pH 9.5 about 60% of maximal activity Homo sapiens