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Literature summary extracted from

  • Chaudhuri, P.; Basu, A.; Sengupta, S.; Lahiri, S.; Dutta, T.; Ghosh, A.K.
    Studies on substrate specificity and activity regulating factors of trehalose-6-phosphate synthase of Saccharomyces cerevisiae (2009), Biochim. Biophys. Acta, 1790, 368-374.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 CDTA 2.5 mM, 134% of initial activity Saccharomyces cerevisiae
2.4.1.15 chondroitin stimulation, particularly when a pyrimidine glucose nucleotide is used, rather than a purine glucose nucleotide Saccharomyces cerevisiae
2.4.1.15 EDTA 2.5 mM, 121% of initial activity Saccharomyces cerevisiae
2.4.1.15 heparin stimulation, particularly when a pyrimidine glucose nucleotide is used, rather than a purine glucose nucleotide. Heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae
2.4.1.15 iodoacetamide 1 mM, 245% of initial activity Saccharomyces cerevisiae
2.4.1.15 iodoacetic acid 0.1 mM, 185% of initial activity Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.4.1.15 heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 Citric acid 2.5 mM, 82% residual activity Saccharomyces cerevisiae
2.4.1.15 N-ethylmaleimide 1 mM, 83% residual activity Saccharomyces cerevisiae
2.4.1.15 Sodium azide 2.5 mM, 83% residual activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 2
-
 UDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
2.4.1.15 2.17
-
 GDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
2.4.1.15 2.38
-
 TDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
2.4.1.15 4.5
-
 D-glucose 6-phosphate pH 8.5, 37°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 MnCl2 stimulation Saccharomyces cerevisiae
2.4.1.15 ZnCl2 stimulation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.15 glycoprotein protein/sugar ratio is 10.75 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.15 1.48
-
 pH 8.5, 37°C Saccharomyces cerevisiae
2.4.1.15 1.58
-
 pH 8.5, 37°C, presence of heparin Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 GDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae GDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
2.4.1.15 TDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae TDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-fructose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-mannose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 40
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.15
-
 40 stable within Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 8.5
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.15 4.5
-
 40 min, 19% residual activity Saccharomyces cerevisiae
2.4.1.15 8.5
-
 stable at alkaline pH values, 100% stability at pH 8.5 Saccharomyces cerevisiae