EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.39 | overexpressed | Paraburkholderia xenovorans LB400 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.2.52 | the active site of HpaI is formed by approx. 30 residues from adjacent dimers and consists of an approx. 15 A deep bell-shaped cleft with an approx. 12 A wide mouth. This broad entrance to the active site is predominantly lined with noncharged residues and a few positively charged residues | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.52 | 2-oxobutanoate | competitive inhibition | Escherichia coli | |
4.1.2.52 | 2-oxopentanoate | competitive inhibition | Escherichia coli | |
4.1.2.52 | 4-methyl-2-oxopentanoate | competitive inhibition | Escherichia coli | |
4.1.2.52 | glyoxylate | competitive inhibition | Escherichia coli | |
4.1.2.52 | pyruvate | competitive inhibition | Escherichia coli | |
4.1.3.39 | 2-ketobutanoate | - |
Escherichia coli | |
4.1.3.39 | 2-ketobutanoate | - |
Paraburkholderia xenovorans | |
4.1.3.39 | 2-ketobutanoate | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 2-ketopentanoate | - |
Escherichia coli | |
4.1.3.39 | 2-ketopentanoate | - |
Paraburkholderia xenovorans | |
4.1.3.39 | 2-ketopentanoate | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 4-methyl-2-oxopentanoate | - |
Escherichia coli | |
4.1.3.39 | 4-methyl-2-oxopentanoate | - |
Paraburkholderia xenovorans | |
4.1.3.39 | 4-methyl-2-oxopentanoate | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.39 | glyoxylate | - |
Escherichia coli | |
4.1.3.39 | glyoxylate | - |
Paraburkholderia xenovorans | |
4.1.3.39 | glyoxylate | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.39 | pyruvate | - |
Escherichia coli | |
4.1.3.39 | pyruvate | - |
Paraburkholderia xenovorans | |
4.1.3.39 | pyruvate | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.43 | 2-oxobutanoate | competitive inhibition | Paraburkholderia xenovorans | |
4.1.3.43 | 2-oxopentanoate | competitive inhibition | Paraburkholderia xenovorans | |
4.1.3.43 | 4-methyl-2-oxopentanoate | competitive inhibition | Paraburkholderia xenovorans | |
4.1.3.43 | glyoxylate | competitive inhibition | Paraburkholderia xenovorans | |
4.1.3.43 | pyruvate | competitive inhibition | Paraburkholderia xenovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.52 | 5.6 | - |
pyruvate | steady-state kinetic parameter, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 9.1 | - |
Succinic semialdehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 13.4 | - |
Butyraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 32.9 | - |
propionaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 33.3 | - |
glycolaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 50.1 | - |
acetaldehyde | Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 62.1 | - |
acetaldehyde | steady-state kinetic parameter, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 62.9 | - |
acetaldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 73.8 | - |
Isobutyraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 88.6 | - |
DL-glyceraldehyde | Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.3.39 | 13.4 | - |
Butyraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 32.9 | - |
propionaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 33.3 | - |
glycolaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 62.9 | - |
acetaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 64.28 | - |
acetaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 64.8 | - |
acetaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 73.8 | - |
Succinic semialdehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 88.6 | - |
(R,S)-glyceraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 124.6 | - |
glycolaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 124.6 | - |
glycolaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 135.9 | - |
propionaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 135.9 | - |
propionaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.43 | 14.8 | - |
pyruvate | steady-state kinetic parameter, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 64.3 | - |
acetaldehyde | app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 67 | - |
acetaldehyde | steady-state kinetic parameter, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 124.6 | - |
glycolaldehyde | app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 135.9 | - |
propionaldehyde | app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.52 | Co2+ | - |
Escherichia coli | |
4.1.3.39 | Mn2+ | - |
Paraburkholderia xenovorans LB400 | |
4.1.3.43 | Mn2+ | cofactor | Paraburkholderia xenovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.52 | pyruvate + succinic semialdehyde | Escherichia coli | - |
4-hydroxy-2-oxo-1,7-heptanedioate | enzyme lacks stereospecific control producing racemic mixtures of its physiological substrate, 4-hydroxy-2-oxo-1,7-heptanedioate | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.52 | Escherichia coli | - |
- |
- |
4.1.3.39 | Escherichia coli | Q47098 | - |
- |
4.1.3.39 | Paraburkholderia xenovorans | - |
- |
- |
4.1.3.39 | Paraburkholderia xenovorans LB400 | P51015 | - |
- |
4.1.3.43 | Paraburkholderia xenovorans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.39 | - |
Paraburkholderia xenovorans LB400 |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.1.2.52 | 4-hydroxy-2-oxoheptanedioate = pyruvate + succinate semialdehyde | rapid equilibrium random order mechanism | Escherichia coli | |
4.1.3.39 | (S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate | rapid equilibrium random order mechanism | Escherichia coli | |
4.1.3.39 | (S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate | compulsory order mechanism, with pyruvate binding first | Paraburkholderia xenovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.52 | 2-oxobutanoate + acetaldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + acetaldehyde | - |
Escherichia coli | 4-hydroxy-2-oxopentanoate | enzyme lacks stereospecific control producing racemic mixtures of 4-hydroxy-2-oxopentanoate i.e. HOPA | ? | |
4.1.2.52 | pyruvate + butyraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + DL-glyceraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + glycolaldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + isobutyraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + pentaldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + propionaldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.2.52 | pyruvate + succinic semialdehyde | - |
Escherichia coli | 4-hydroxy-2-oxo-1,7-heptanedioate | enzyme lacks stereospecific control producing racemic mixtures of its physiological substrate, 4-hydroxy-2-oxo-1,7-heptanedioate | ? | |
4.1.3.39 | additional information | BphI exhibits a compulsory order mechanism, with pyruvate binding first. BphI is able to utilize acetaldehyde produced by the reduction of acetyl-CoA catalyzed by the associated aldehyde dehydrogenase, BphJ. This aldehyde is directly channeled from the dehydrogenase to the aldolase active sites, with an efficiency of 84%. The BphJ reductive deacylation reaction increases 4fold when BphI is catalyzing the aldol addition reaction. The BphI-BphJ enzyme complex exhibits unique bidirectionality in substrate channeling and allosteric activation | Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | additional information | BphI is able to utilize aldehyde acceptors two to five carbons in length. BphI is able to bind 2-keto acids larger than pyruvate. BphI synthesizes only (4S)-4-hydroxy-2-oxopentanoate from pyruvate and acetaldehyde. BphI is also able to utilize acetaldehyde produced by the reduction of acetyl-CoA catalyzed by the associated aldehyde dehydrogenase, BphJ. This aldehyde is directly channeled from the dehydrogenase to the aldolase active sites, with an efficiency of 84%. The BphJ reductive deacylation reaction increases 4-fold when BphI is catalyzing the aldol addition reaction. The BphI-BphJ enzyme complex exhibits unique bidirectionality in substrate channeling and allosteric activation | Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.39 | additional information | HpaI is able to utilize aldehyde acceptors two to five carbons in length, shows broad specificity and has a preference for aldehydes containing longer linear alkyl chains or C2-OH substitutions. HpaI is able to bind 2-keto acids larger than pyruvate, and to utilize both pyruvate and 2-ketobutanoate as carbonyl donors in the aldol addition reaction. HpaI lacks stereospecific control producing racemic mixtures of 4-hydroxy-2-oxopentanoate from pyruvate and acetaldehyde | Escherichia coli | ? | - |
? | |
4.1.3.39 | pyruvate + (R,S)-glyceraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.3.39 | pyruvate + acetaldehyde | - |
Paraburkholderia xenovorans LB400 | (4S)-hydroxy-2-oxopentanoate | - |
? | |
4.1.3.39 | pyruvate + acetaldehyde | - |
Paraburkholderia xenovorans | (4S)-4-hydroxy-2-oxovalerate | - |
? | |
4.1.3.39 | pyruvate + acetaldehyde | - |
Escherichia coli | 4-hydroxy-2-oxopentanoate | - |
? | |
4.1.3.39 | pyruvate + butyraldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.39 | pyruvate + butyraldehyde | - |
Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | pyruvate + butyraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.3.39 | pyruvate + glycolaldehyde | - |
Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | pyruvate + glycolaldehyde | - |
Paraburkholderia xenovorans | 4,5-dihydroxy-2-oxo-pentanoic acid | - |
? | |
4.1.3.39 | pyruvate + glycolaldehyde | - |
Escherichia coli | 4,5-dihydroxy-2-oxo-pentanoic acid | - |
? | |
4.1.3.39 | pyruvate + isobutyraldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.39 | pyruvate + isobutyraldehyde | - |
Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | pyruvate + isobutyraldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.3.39 | pyruvate + pentaldehyde | - |
Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | pyruvate + propionaldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.39 | pyruvate + propionaldehyde | - |
Paraburkholderia xenovorans LB400 | ? | - |
? | |
4.1.3.39 | pyruvate + propionaldehyde | - |
Escherichia coli | ? | - |
? | |
4.1.3.39 | pyruvate + succinic semialdehyde | - |
Escherichia coli | (4RS)-4-hydroxy-2-oxoheptane-1,7-dioate | racemic product | ? | |
4.1.3.39 | pyruvate + succinic semialdehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.43 | pyruvate + acetaldehyde | - |
Paraburkholderia xenovorans | (4S)-4-hydroxy-2-oxopentanoate | - |
? | |
4.1.3.43 | pyruvate + butyraldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.43 | pyruvate + glycolaldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.43 | pyruvate + isobutyraldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.43 | pyruvate + pentaldehyde | - |
Paraburkholderia xenovorans | ? | - |
? | |
4.1.3.43 | pyruvate + propionaldehyde | - |
Paraburkholderia xenovorans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.52 | HpaI | - |
Escherichia coli |
4.1.3.39 | BphI | - |
Paraburkholderia xenovorans |
4.1.3.39 | BphI | - |
Paraburkholderia xenovorans LB400 |
4.1.3.39 | pyruvate aldolase | - |
Paraburkholderia xenovorans LB400 |
4.1.3.43 | BphI | - |
Paraburkholderia xenovorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.52 | 5.6 | - |
DL-glyceraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 21.9 | - |
acetaldehyde | Kcat(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 64.9 | - |
Isobutyraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 132.5 | - |
Butyraldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 175.5 | - |
glycolaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 203.8 | - |
Succinic semialdehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 205.4 | - |
acetaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 219.5 | - |
pyruvate | steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 358.4 | - |
propionaldehyde | Kcat(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.3.39 | 0.4 | - |
glycolaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 0.4 | - |
glycolaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.86 | - |
acetaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 0.86 | - |
acetaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 1.79 | - |
propionaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 1.79 | - |
propionaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 5.6 | - |
(R,S)-glyceraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 64.9 | - |
Succinic semialdehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 132.5 | - |
Butyraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 175.5 | - |
glycolaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 205.4 | - |
acetaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 358.4 | - |
propionaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.43 | 0.4 | - |
glycolaldehyde | app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.86 | - |
acetaldehyde | app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 1.04 | - |
pyruvate | steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 1.79 | - |
propionaldehyde | app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.52 | 0.4 | - |
glyoxylate | pH 8.0, 25°C | Escherichia coli | |
4.1.2.52 | 0.5 | - |
2-oxobutanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.2.52 | 0.51 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
4.1.2.52 | 2.01 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
4.1.2.52 | 3.6 | - |
2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.2.52 | 6.98 | - |
4-methyl-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 0.21 | - |
glyoxylate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 0.21 | - |
glyoxylate | in the presence of 0.4 mM NADH, 2 mM Mn2+, 20 units of ADH in 100 mM HEPES buffer, pH 8.0 at 25°C, in a total volume of 1 ml | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.34 | - |
2-ketobutanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 0.34 | - |
2-ketobutanoate | in the presence of 0.4 mM NADH, 2 mM Mn2+, 20 units of ADH in 100 mM HEPES buffer, pH 8.0 at 25°C, in a total volume of 1 ml | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.4 | - |
glyoxylate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 0.5 | - |
2-ketobutanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 0.51 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 0.55 | - |
pyruvate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 0.55 | - |
pyruvate | in the presence of 0.4 mM NADH, 2 mM Mn2+, 20 units of ADH in 100 mM HEPES buffer, pH 8.0 at 25°C, in a total volume of 1 ml | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 1.09 | - |
2-ketopentanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 1.09 | - |
2-ketopentanoate | in the presence of 0.4 mM NADH, 2 mM Mn2+, 20 units of ADH in 100 mM HEPES buffer, pH 8.0 at 25°C, in a total volume of 1 ml | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 2.71 | - |
4-methyl-2-oxopentanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 2.71 | - |
4-methyl-2-oxopentanoate | in the presence of 0.4 mM NADH, 2 mM Mn2+, 20 units of ADH in 100 mM HEPES buffer, pH 8.0 at 25°C, in a total volume of 1 ml | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 3.6 | - |
2-ketopentanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 6.98 | - |
4-methyl-2-oxopentanoate | pH 8.0, 25°C | Escherichia coli | |
4.1.3.43 | 0.21 | - |
glyoxylate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.34 | - |
2-oxobutanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.55 | - |
pyruvate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 1.09 | - |
2-oxopentanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 2.71 | - |
4-methyl-2-oxopentanoate | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 10.3 | - |
pyruvate | pH 8.0, 25°C | Paraburkholderia xenovorans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.52 | 0.063 | - |
DL-glyceraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 0.4 | - |
acetaldehyde | Kcat/Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 0.9 | - |
Isobutyraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 3.3 | - |
acetaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 5.27 | - |
glycolaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 9.9 | - |
Butyraldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 10.9 | - |
propionaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 20 | - |
pentaldehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.2.52 | 22.2 | - |
Succinic semialdehyde | Kcat/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Escherichia coli | |
4.1.3.39 | 0.00047 | - |
pentaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.0005 | - |
Isobutyraldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.00219 | - |
Butyraldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.0037 | - |
glycolaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.0132 | - |
propionaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.0134 | - |
acetaldehyde | at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate | Paraburkholderia xenovorans LB400 | |
4.1.3.39 | 0.063 | - |
(R,S)-glyceraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 0.5 | - |
Succinic semialdehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 2.19 | - |
Butyraldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 3.3 | - |
acetaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 3.7 | - |
glycolaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 5.27 | - |
glycolaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 9.9 | - |
Butyraldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 10.9 | - |
propionaldehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.39 | 13.2 | - |
propionaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 13.4 | - |
acetaldehyde | pH 8.0, 25°C | Paraburkholderia xenovorans | |
4.1.3.39 | 22.5 | - |
Succinic semialdehyde | pH 8.0, 25°C | Escherichia coli | |
4.1.3.43 | 0.00047 | - |
pentaldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.0005 | - |
Isobutyraldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.0022 | - |
Butyraldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.0037 | - |
glycolaldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.0132 | - |
propionaldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans | |
4.1.3.43 | 0.0134 | - |
acetaldehyde | kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C | Paraburkholderia xenovorans |