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Literature summary extracted from

  • Ziebart, K.T.; Toney, M.D.
    Nucleophile specificity in anthranilate synthase, aminodeoxychorismate synthase, isochorismate synthase, and salicylate synthase (2010), Biochemistry, 49, 2851-2859.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.85
-
 Escherichia coli
4.1.3.27 wild-type and mutant overexpressed in Escherichia coli cells (strain CB694) harboring the pSTC25 plasmid Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.85 K213A subunit PabB, numbering used is based on the EntC protein from Escherichia coli, reduced activity Escherichia coli
2.6.1.85 K213A/Q147K subunit PabB, numbering used is based on the EntC protein from Escherichia coli, strongly reduced activity Escherichia coli
4.1.3.27 Q147K does not exhibit anthranilate synthase activity. Using (NH4)2SO4, 29% of the wild-type activity is restored to the mutant, with anthranilate representing 97% of all products. Employs water as a nucleophile to a small extent Salmonella enterica subsp. enterica serovar Typhimurium
4.2.99.21 K147Q mutation in proposed catalytic base, about 50fold decrease in activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 2.5
-
 L-glutamine wild-type, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
4.1.3.27 3.6
-
 L-glutamine mutant Q147K, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Escherichia coli
-
-
-
4.1.3.27 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
4.2.99.21 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.85
-
 Escherichia coli
4.1.3.27 wild-type and mutant purified by hydroxyapatite chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
4.1.3.27 -80°C, 20 mM KH2PO4 buffer, 50 mM KCl, 1 mM DTT, pH 7.5 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-glutamine
-
 Escherichia coli L-glutamate + 4-amino-4-deoxychorismate
-
 ?
4.1.3.27 chorismate + L-glutamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH3
-
 Salmonella enterica subsp. enterica serovar Typhimurium anthranilate + pyruvate + H2O
-
 ?
4.1.3.27 additional information does not possess chorismate mutase promiscuous activity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
4.2.99.21 additional information nucleophilic substitution reaction, enzyme is able to use H2O as a nucleophile. Catalytic base K147 is not solely responsible for activation of H2O as a nucleophile Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.85 heterodimer PabA-PabB Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.85 aminodeoxychorismate synthase
-
 Escherichia coli
4.1.3.27 anthranilate synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.85 0.6
-
 L-glutamine mutant K213A/Q147K amidotransferase activity, pH 8.0, 25°C Escherichia coli
2.6.1.85 0.67
-
 L-glutamine wild type amidotransferase activity, pH 8.0, 25°C Escherichia coli
4.1.3.27 138
-
 L-glutamine wild-type, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
4.1.3.27 152
-
 L-glutamine mutant Q147K, at 25°C, in 100 mM phosphate buffer, pH 7.0, with 20 mM L-glutamine and 5 mM MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium