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Literature summary extracted from

  • Wecksler, A.T.; Jacquot, C.; van der Donk, W.A.; Holman, T.R.
    Mechanistic investigations of human reticulocyte 15- and platelet 12-lipoxygenases with arachidonic acid (2009), Biochemistry, 48, 6259-6267.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.33 12(S)-hydroperoxyeicosatetraenoic acid reduces the kinetic lag phase Homo sapiens
1.13.11.33 13(S)-hydroperoxyoctadecadienoic acid activates 15-hLO-1 and removes the kinetic lag phase Homo sapiens
1.13.11.33 15(S)-hydroperoxyeicosatetraenoic acid activates 15-hLO-1 and removes the kinetic lag phase Homo sapiens
1.13.11.33 additional information under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantly, tunneling mechanism Homo sapiens

Application

EC Number Application Comment Organism
1.13.11.33 medicine under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantlysignificantly, which may have important implications in cancer progression Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 overexpressed as N-terminally, His6-tagged protein Homo sapiens
1.13.11.33 overexpressed as N-terminally, His6-tagged protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.33 arachidonic acid autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations Homo sapiens
1.13.11.33 linoleic acid autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.33 0.0096
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
1.13.11.33 0.015
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 0.022
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 0.024
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.31 Fe2+
-
 Homo sapiens
1.13.11.33 Fe2+ iron-containing metalloenzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
-
-
1.13.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 to greater than 90% purity Homo sapiens
1.13.11.33 to greater than 90% purity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 blood platelet
-
 Homo sapiens
-
1.13.11.33 reticulocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
 Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.33 arachidonate + O2 5fold higher affinity for arachidonic acid than for linoleic acid for 15-hLO-1 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 linoleic acid + O2
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-lipoxygenase
-
 Homo sapiens
1.13.11.31 12-LO
-
 Homo sapiens
1.13.11.33 15-hLO-1
-
 Homo sapiens
1.13.11.33 15-lipoxygenase-1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.33 30
-
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.31 1.4
-
 arachidonic acid at 15°C, pH 7.5 Homo sapiens
1.13.11.31 20
-
 arachidonic acid at 40°C, pH 7.5 Homo sapiens
1.13.11.33 5.1
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 5.6
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
1.13.11.33 7
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 7.6
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.31 1.4
-
 arachidonic acid at 15°C, pH 7.5 Homo sapiens
1.13.11.31 50
-
 arachidonic acid at 40°C, pH 7.5 Homo sapiens
1.13.11.33 240
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
1.13.11.33 320
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 340
-
 O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
1.13.11.33 790
-
 O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens