EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.62 | into the vector pT7-7 for expression in Escherichia coli BL21-CodonPlus DE3 cells | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.62 | 0.0024 | - |
GTP | apparent KM | Methanocaldococcus jannaschii | |
2.7.7.62 | 0.0184 | - |
adenosylcobinamide phosphate | apparent KM | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.62 | 21600 | - |
monomer, determined by gel filtation and SDS-PAGE | Methanocaldococcus jannaschii |
2.7.7.62 | 43000 | - |
dimer, determined by gel filtation | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.62 | GTP + adenosylcobinamide phosphate | Methanocaldococcus jannaschii | - |
diphosphate + adenosylcobinamide-GDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.62 | Methanocaldococcus jannaschii | Q58517 | MJ1117 open reading frame | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.62 | in two steps by hydrophobic interaction and ion-exchange chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.62 | GTP + adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii | diphosphate + adenosylcobinamide-GDP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.62 | homodimer | 2 * 21600 | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.62 | CobY | - |
Methanocaldococcus jannaschii |
2.7.7.62 | GTP:adenosylcobinamide-phosphate guanylyltransferase | - |
Methanocaldococcus jannaschii |
2.7.7.62 | MjCobY | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.62 | 37 | - |
kinetic assay | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.62 | 80 | - |
MjCobY denaturates at 80°C with a concomitant loss of activity | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.62 | 1.8 | - |
GTP | - |
Methanocaldococcus jannaschii | |
2.7.7.62 | 4.7 | - |
adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii |