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Literature summary extracted from
- The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy (2009), Biochemistry, 48, 5042-5049.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | heterologous expression and synthesis of [FeFe] hydrogenase in Clostridium acetobutylicum ATCC 824 | Chlamydomonas reinhardtii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | CO | bond length alterations after incubation of HydA1 with CO and H2, related to structural and oxidation state changes at the catalytic Fe atoms, e.g., to the binding of an exogenous CO at 2FeH in CO-inhibited enzyme | Chlamydomonas reinhardtii |  |
| 1.12.99.6 | additional information | loss of Fe-Fe distances in protein purified under mildly oxidizing conditions, partial degradation of the H-cluster | Chlamydomonas reinhardtii |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Fe | the HydA1 H-cluster consists of a [4Fe4S] cluster and a diiron site, 2FeH | Chlamydomonas reinhardtii | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | purification of the protein under strictly anaerobic and reducing conditions to obtain the as-isolated state of HydA1 | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 40 | - |
CO treated | Chlamydomonas reinhardtii |
| 1.12.99.6 | 590 | - |
HydA1ox | Chlamydomonas reinhardtii |
| 1.12.99.6 | 620 | - |
H2 treated | Chlamydomonas reinhardtii |
| 1.12.99.6 | 650 | - |
HydA1red | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + oxidized electron acceptor | - |
Chlamydomonas reinhardtii | H+ + reduced electron acceptor | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | HydA1 | - |
Chlamydomonas reinhardtii |
| 1.12.99.6 | [FeFe] hydrogenase | - |
Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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