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Literature summary extracted from

  • Reyda, M.R.; Fugate, C.J.; Jarrett, J.T.
    A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly (2009), Biochemistry, 48, 10782-10792.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6 hexahistidine tagged protein is expressed in Escherichia coli cells Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 45000
-
 His6-BioB, determined by SDS-PAGE and Western blot analysis Escherichia coli
2.8.1.6 82630
-
 homodimer, theoretical Escherichia coli
2.8.1.6 90680
-
 homodimer, determined by analytical ultracentrifugation Escherichia coli
2.8.1.6 165300
-
 homotetramer, theoretical Escherichia coli
2.8.1.6 173900
-
 homotetramer, determined by analytical ultracentrifugation, non-physiological form Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + S-adenosyl-L-methionine Escherichia coli
-
 biotin + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6 His6-BioB is purified from Escherichia coli K12 WT strain BW25113, or from knockout strains lacking HscA, HscB, or DnaK, purification on a Ni-NTA-agarose column Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + S-adenosyl-L-methionine
-
 Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 homodimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.8.1.6 BioB
-
 Escherichia coli
2.8.1.6 biotin synthase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.6 iron-sulfur centre
-
 Escherichia coli
2.8.1.6 S-adenosyl-L-methionine
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.8.1.6 physiological function biotin synthase catalyses the last step in the biosynthesis of biotin Escherichia coli