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Literature summary extracted from

  • Vetting, M.W.; Park, C.H.; Hegde, S.S.; Jacoby, G.A.; Hooper, D.C.; Blanchard, J.S.
    Mechanistic and structural analysis of aminoglycoside N-acetyltransferase AAC(6)-Ib and its bifunctional, fluoroquinolone-active AAC(6)-Ib-cr variant (2008), Biochemistry, 47, 9825-9835.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.82 the structure of AAC(6')-Ib is determined in various complexes with donor and acceptor ligands to resolutions greater than 2.2 A Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.82 lividomycin A
-
 Escherichia coli
2.3.1.82 Pefloxacin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.00027
-
 kanamycin B AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 0.0053
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 0.0067
-
 kanamycin B AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 0.02
-
 neomycin B AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 0.035
-
 acetyl-CoA AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 0.067
-
 Norfloxacin AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 0.07
-
 Ciprofloxacin AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 0.108
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 on a Ni-NTA and a Superdex S-75 column, the His6 tag is cleaved using thrombin Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + ciprofloxacin
-
 Escherichia coli CoA + N4'-acetylnorfloxacin
-
 ?
2.3.1.82 acetyl-CoA + kanamycin B
-
 Escherichia coli CoA + N6'-acetylkanamycin B
-
 ?
2.3.1.82 acetyl-CoA + neomycin B
-
 Escherichia coli CoA + N6'-acetylneomycin B
-
 ?
2.3.1.82 acetyl-CoA + norfloxacin
-
 Escherichia coli CoA + N4'-acetylnorfloxacin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(6')-Ib
-
 Escherichia coli
2.3.1.82 AAC(6')-Ib-cr bifunctional variant of AAC(6')-Ib Escherichia coli
2.3.1.82 aminoglycoside N-acetyltransferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 1.03
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 1.03
-
 Ciprofloxacin AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 1.08
-
 Norfloxacin AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 2.27
-
 kanamycin B AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 2.32
-
 acetyl-CoA AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 3.08
-
 neomycin B AAC(6')-Ib, wild type Escherichia coli
2.3.1.82 5.23
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
2.3.1.82 5.25
-
 kanamycin B AAC(6')-Ib-cr variant Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.82 5.6
-
 AAC(6')-Ib Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.82 acetyl-CoA
-
 Escherichia coli