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Literature summary extracted from

  • Jeng, I.M.; Somack, R.; Barker, H.A.
    Ornithine degradation in Clostridium sticklandii: pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4-ketopentanoate to alanine and acetyl-CoA (1974), Biochemistry, 13, 2989-2903.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.263 additional information the enzyme is completely inactivated by dialysis against 100 mM cysteine and can be reactivated by addition of pyridoxal 5'-phosphate Acetoanaerobium sticklandii
2.3.1.263 N-ethylmaleimide
-
 Acetoanaerobium sticklandii
2.3.1.263 PCMB
-
 Acetoanaerobium sticklandii
2.3.1.263 pyridoxal
-
 Acetoanaerobium sticklandii
2.3.1.263 pyridoxamine 5'-phosphate
-
 Acetoanaerobium sticklandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.263 0.00025
-
 CoA pH 8.0, 26°C Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.263 CoA + 2-amino-4-ketopentanoate Clostridium subterminale catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 r
2.3.1.263 CoA + 2-amino-4-ketopentanoate Clostridium subterminale SB4 catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 r
2.3.1.263 CoA + 2-amino-4-oxopentanoate + CoA Acetoanaerobium sticklandii catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.263 Acetoanaerobium sticklandii
-
-
-
2.3.1.263 Clostridium subterminale
-
 specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-
2.3.1.263 Clostridium subterminale SB4
-
 specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.263 partial Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
2.3.1.263 -20°C, thiolase activity in crude extract remains constant for at least 10 days Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.263 CoA + 2-amino-4-ketopentanoate catalyzes a step in the ornithine fermentation pathway Clostridium subterminale acetyl-CoA + D-alanine
-
 r
2.3.1.263 CoA + 2-amino-4-ketopentanoate catalyzes a step in the ornithine fermentation pathway Clostridium subterminale SB4 acetyl-CoA + D-alanine
-
 r
2.3.1.263 CoA + 2-amino-4-oxopentanoate
-
 Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 ?
2.3.1.263 CoA + 2-amino-4-oxopentanoate
-
 Clostridium subterminale acetyl-CoA + D-alanine
-
 ?
2.3.1.263 CoA + 2-amino-4-oxopentanoate
-
 Clostridium subterminale SB4 acetyl-CoA + D-alanine
-
 ?
2.3.1.263 CoA + 2-amino-4-oxopentanoate + CoA catalyzes a step in the ornithine fermentation pathway Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.263 AKP-thiolase
-
 Acetoanaerobium sticklandii
2.3.1.263 AKP-thiolase
-
 Clostridium subterminale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.263 24
-
 thiolase activity in crude extract remains constant for at least 2 days Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.263 pyridoxal 5'-phosphate Km: 0.00025 mM. Strictly pyridoxal 5'-phosphate-dependent enzyme. The enzyme is completely inactivated by dialysis against 100 mM cysteine and can be reactivated by addition of pyridoxal 5'-phosphate. Other pyridoxal phosphate analogs and alpha-keto acids cannot replace pyridoxal 5'-phosphate Acetoanaerobium sticklandii