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Literature summary extracted from

  • Rosenzweig, A.C.
    The metal centres of particulate methane mono-oxygenase (2008), Biochem. Soc. Trans., 36, 1134-1137.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.3 to 2.8 A resolution Methylococcus capsulatus
1.14.18.3 to 3.9 A resolution Methylosinus trichosporium OB3b

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 copper copper contents per 100 kDa alphabetagamma protomer of 15-20, eight to ten, two to three and two. Mixture of Cu(I) and Cu(II), copper cluster with a short Cu-Cu distance of 2.51 A that increases to 2.65 A upon chemical reduction with dithionite. The pMMO contains a mononuclear type 2Cu(II) centre and some type of copper cluster Methylococcus capsulatus
1.14.18.3 copper contains approximately two copper ions per 100 kDa protomer, type 2 Cu(II) present as two distinct species, mixture of Cu(I) and Cu(II). Short Cu-Cu interaction at 2.51 A. Di-copper centre plays an important functional role in pMMO, whereas the mononuclear copper centre is not critical Methylosinus trichosporium OB3b
1.14.18.3 Fe2+ 0.75 to two iron ions per protomer. Di-iron may be the pMMO active site Methylococcus capsulatus
1.14.18.3 additional information no detectable iron Methylosinus trichosporium OB3b
1.14.18.3 Zinc site within the membrane that can be occupied by zinc or copper Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane
-
 Methylosinus trichosporium OB3b 16020
-
1.14.18.3 membrane
-
 Methylococcus capsulatus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus G1UBD1
-
-
1.14.18.3 Methylococcus capsulatus Bath G1UBD1
-
-
1.14.18.3 Methylosinus trichosporium OB3b
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3
-
 Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus Bath methanol + acceptor + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 particulate methane mono-oxygenase
-
 Methylosinus trichosporium OB3b
1.14.18.3 particulate methane mono-oxygenase
-
 Methylococcus capsulatus
1.14.18.3 pMMO
-
 Methylosinus trichosporium OB3b
1.14.18.3 pMMO
-
 Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
 Methylococcus capsulatus
1.14.18.3 NADPH
-
 Methylococcus capsulatus