EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.37 | M.HhaI overexpression in Escherichia coli strain ER1727 | Haemophilus parahaemolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.37 | 2-(1H)-pyrimidinone riboside | i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction | Haemophilus parahaemolyticus | |
2.1.1.37 | 5-azacytidine | zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds | Haemophilus parahaemolyticus | |
2.1.1.37 | additional information | inhibitor binding kinetics | Haemophilus parahaemolyticus | |
2.1.1.37 | oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone | oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview | Haemophilus parahaemolyticus | |
2.1.1.37 | oligodeoxyribonucleotides containing 5-azacytidine | 5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview | Haemophilus parahaemolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.37 | DNA + S-adenosyl-L-methionine | Haemophilus parahaemolyticus | - |
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.37 | Haemophilus parahaemolyticus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.37 | DNA + S-adenosyl-L-methionine | - |
Haemophilus parahaemolyticus | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.37 | DNA (cytosine-C5) methyltransferase | - |
Haemophilus parahaemolyticus |
2.1.1.37 | M.HhaI | - |
Haemophilus parahaemolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.37 | 37 | - |
assay at | Haemophilus parahaemolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.37 | 7.5 | - |
assay at | Haemophilus parahaemolyticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.37 | S-adenosyl-L-methionine | the ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction | Haemophilus parahaemolyticus |