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Literature summary extracted from

  • Gisk, B.; Yasui, Y.; Kohchi, T.; Frankenberg-Dinkel, N.
    Characterization of the heme oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions (2010), Biochem. J., 425, 425-434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.18 ascorbate addition of a second electron donor like ascorbate leads to 10times faster heme conversion Arabidopsis thaliana
1.14.14.18 additional information heme turnover is promoted by light when spinach thylakoids are present Arabidopsis thaliana
1.14.15.20 L-ascorbate adding of a second electron donor, such as ascorbate, led to a 10fold increase in the heme conversion rate Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.18 HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260 Arabidopsis thaliana
1.14.15.20 expressed in Nicotiana benthamiana and Escherichia coli BL21 (DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.18 0.0027
-
 heme HO3, without ascorbate Arabidopsis thaliana
1.14.14.18 0.0057
-
 heme HO4, without ascorbate Arabidopsis thaliana
1.14.15.20 0.0013
-
 protoheme isoform HY1, at pH 7.2 and 25°C Arabidopsis thaliana
1.14.15.20 0.0027
-
 protoheme isoform HO3, at pH 7.2 and 25°C Arabidopsis thaliana
1.14.15.20 0.0057
-
 protoheme isoform HO4, at pH 7.2 and 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.18 chloroplast HY1, HO3, and HO4 are present as the processed mature protein in the plastid compartment Arabidopsis thaliana 9507
-
1.14.14.18 additional information no mitochondrial localization of HY1, HO3, and HO4 Arabidopsis thaliana
-
-
1.14.15.20 chloroplast
-
 Arabidopsis thaliana 9507
-
1.14.15.20 nucleus
-
 Arabidopsis thaliana 5634
-
1.14.15.20 plastid isoforms HY1, HO3 and HO4 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.18 27000
-
 1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Arabidopsis thaliana
-
 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.18 Arabidopsis thaliana
-
-
-
1.14.15.20 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.18 HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity Arabidopsis thaliana
1.14.15.20 Talon-cobalt-affinity column chromatography Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
1.14.14.18 0°C, 100 mM potassium phosphate buffer, pH 7.2, 4 days Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.18 heme + ferredoxin + O2 HY1, HO3, and HO4 Arabidopsis thaliana biliverdin IXalpha + Fe2+ + CO + A + H2O
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Arabidopsis thaliana biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.18 monomer 1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration Arabidopsis thaliana
1.14.15.20 monomer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.14.18 Ho3
-
 Arabidopsis thaliana
1.14.14.18 HO4
-
 Arabidopsis thaliana
1.14.14.18 HY1 HO1 Arabidopsis thaliana
1.14.15.20 haem oxygenase
-
 Arabidopsis thaliana
1.14.15.20 Ho1
-
 Arabidopsis thaliana
1.14.15.20 Ho3
-
 Arabidopsis thaliana
1.14.15.20 HO4
-
 Arabidopsis thaliana
1.14.15.20 HY1
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.14.18 10 45
-
 Arabidopsis thaliana
1.14.15.20 10 45 the relative enzyme activity increases between 10°C and 45°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.18 7
-
 HO3 Arabidopsis thaliana
1.14.14.18 7.25
-
 HY1 and HO4 Arabidopsis thaliana
1.14.15.20 7
-
 isoform HO3 Arabidopsis thaliana
1.14.15.20 7.3
-
 isoforms HO4 and HY1 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.18 heme
-
 Arabidopsis thaliana
1.14.14.18 NADPH
-
 Arabidopsis thaliana