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Literature summary extracted from

  • Alberge, B.; Gannoun-Zaki, L.; Bascunana, C.; Tran van Ba, C.; Vial, H.; Cerdan, R.
    Comparison of the cellular and biochemical properties of Plasmodium falciparum choline and ethanolamine kinases (2010), Biochem. J., 425, 149-158.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.82 medicine ethanolamine kinase could be a target for antimalerial therapy Plasmodium falciparum 3D7

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 His-tag, expressed in Escherichia coli BL21(DE3)-pRIL Plasmodium falciparum 3D7
2.7.1.82 expression in Escherichia coli Plasmodium falciparum
2.7.1.82 into the vector pET24b for expression in Escherichia coli BL21DE3-pRIL cells Plasmodium falciparum 3D7

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 2-amino-1-butanol
-
Plasmodium falciparum 3D7
2.7.1.32 hemicholinium-3
-
Plasmodium falciparum 3D7
2.7.1.32 SAR97276
-
Plasmodium falciparum 3D7
2.7.1.82 2-amino-1-butanol analogue of ethanolamine, selective inhibition Plasmodium falciparum
2.7.1.82 2-amino-1-butanol
-
Plasmodium falciparum 3D7
2.7.1.82 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] SAR97276 Plasmodium falciparum 3D7
2.7.1.82 hemicholinium-3
-
Plasmodium falciparum
2.7.1.82 SAR97276 bis-thiazolium compound designed as a choline analogue, affects ethanolamine kinase and choline kinase activities similarly. Inhibition is competitive and correlates with the impairment of cellular phosphatidylcholine biosynthesis Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 additional information
-
ethanolamine above 500 mM Plasmodium falciparum 3D7
2.7.1.32 0.1353
-
choline recombinant protein Plasmodium falciparum 3D7
2.7.1.82 0.458
-
ethanolamine pH 8.0, 37°C Plasmodium falciparum
2.7.1.82 0.4757
-
ethanolamine
-
Plasmodium falciparum 3D7

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.82 cytoplasm
-
Plasmodium falciparum 3D7 5737
-
2.7.1.82 cytoplasm of Plasmodium falciparum Plasmodium falciparum 5737
-
2.7.1.82 additional information protein expression levels increase during erythrocytic development Plasmodium falciparum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 52000
-
1 * 52000, gel filtration Plasmodium falciparum 3D7
2.7.1.82 48000
-
determined by SDS-PAGE and Western Blot analysis Plasmodium falciparum 3D7
2.7.1.82 109000
-
determined by gel filtration Plasmodium falciparum 3D7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.82 ATP + ethanolamine Plasmodium falciparum 3D7
-
ADP + O-phosphoethanolamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Plasmodium falciparum 3D7 Q8IM71
-
-
2.7.1.82 Plasmodium falciparum Q8IIB7 3D7
-
2.7.1.82 Plasmodium falciparum 3D7 Q8IIB7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32
-
Plasmodium falciparum 3D7
2.7.1.82 using a HisTrap chelating and a gel filtration column Plasmodium falciparum 3D7

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.32 additional information
-
62.2 micromol/min/mg for ethanolamine Plasmodium falciparum 3D7
2.7.1.32 10.8
-
recombinant protein Plasmodium falciparum 3D7
2.7.1.82 1.3
-
pH 8.0, 37°C Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 choline + ATP
-
Plasmodium falciparum 3D7 O-phosphocholine + ADP
-
?
2.7.1.82 ATP + ethanolamine
-
Plasmodium falciparum 3D7 ADP + O-phosphoethanolamine
-
?
2.7.1.82 ATP + ethanolamine
-
Plasmodium falciparum ADP + O-phosphoethanolamine
-
?
2.7.1.82 additional information no substrate: choline Plasmodium falciparum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 monomer 1 * 52000, gel filtration Plasmodium falciparum 3D7
2.7.1.82 homodimer
-
Plasmodium falciparum 3D7

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 choline kinase
-
Plasmodium falciparum 3D7
2.7.1.82 ethanolamine kinase
-
Plasmodium falciparum 3D7
2.7.1.82 PfEK
-
Plasmodium falciparum 3D7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.82 37
-
activity assay Plasmodium falciparum 3D7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.82 8
-
activity assay Plasmodium falciparum 3D7

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.82 ATP
-
Plasmodium falciparum 3D7

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.32 0.9
-
SAR97276 calculated from Dixon analysis Plasmodium falciparum 3D7
2.7.1.32 1.03
-
SAR97276 calculated from Lineweaver-Burk analysis Plasmodium falciparum 3D7
2.7.1.82 0.73
-
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
-
Plasmodium falciparum 3D7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.32 0.22
-
endogenous enzyme Plasmodium falciparum 3D7 hemicholinium-3
2.7.1.32 0.52
-
endogenous enzyme Plasmodium falciparum 3D7 SAR97276
2.7.1.32 0.58
-
recombinant enzyme Plasmodium falciparum 3D7 hemicholinium-3
2.7.1.32 1.12
-
recombinant enzyme Plasmodium falciparum 3D7 SAR97276
2.7.1.32 3.2
-
endogenous enzyme Plasmodium falciparum 3D7 2-amino-1-butanol
2.7.1.82 0.46
-
pH 8.0, 37°C Plasmodium falciparum hemicholinium-3
2.7.1.82 0.46
-
Plasmodium falciparum-endogenous ethanolamine kinase Plasmodium falciparum 3D7 2-amino-1-butanol
2.7.1.82 0.61
-
recombinant protein Plasmodium falciparum 3D7 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
2.7.1.82 0.79
-
pH 8.0, 37°C Plasmodium falciparum SAR97276
2.7.1.82 0.79
-
Plasmodium falciparum-endogenous ethanolamine kinase Plasmodium falciparum 3D7 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
2.7.1.82 1.36
-
pH 8.0, 37°C Plasmodium falciparum 2-amino-1-butanol
2.7.1.82 1.36
-
recombinant protein Plasmodium falciparum 3D7 2-amino-1-butanol

Expression

EC Number Organism Comment Expression
2.7.1.82 Plasmodium falciparum 3D7 expression levels increases during erythrocytic development up