EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.21 | - |
Leptospira interrogans |
2.3.3.21 | full-length and mutant CMS | Leptospira interrogans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.3.21 | C-terminal regulatory domain of CMS (CMSC) in complex with isoleucine, by hanging-drop vapour diffusion method. Crystals of CMSC belong to space group C222 (type I) with unit cell parameters a = 62.6 A, b = 98.4 A and c = 40.1 A, refined to 2.5 A resolution. Type II crystals of CMSC grown at room temperature belong to space group C2 with unit cell parameters a = 61.3 A, b = 97.9 A, c = 40.0 A and beta = 91.4, refined to 2.0 A resolution. Type III crystals of CMSC grown at 4°C belong to space group C222 with unit cell parameters a = 108.2 A, b = 118.6 A and c = 63.6 A, refined to 2.7 A resolution. CMSC consists of six beta-strands forming two anti-parallel beta-sheets and two alpha-helices and assumes a betaalphabeta three-layer sandwich structure. The inhibitor isoleucine is bound in a pocket at the dimer interface and has both hydrophobic and hydrogen-bonding interactions with several conserved residues of both subunits | Leptospira interrogans |
2.3.3.21 | hanging-drop vapor diffusion, in 20 mM Tris-HCl, pH 8.4 with 50 mM NaCl | Leptospira interrogans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.21 | D431A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values) | Leptospira interrogans |
2.3.3.21 | D431A | has markedly increased IC50 and Ki values for isoleucine | Leptospira interrogans |
2.3.3.21 | H400A/H408A | prevents Zn2+-binding and enables dimer instead of tetramer formation, no change in enzymatic activity or the inhibition by isoleucine | Leptospira interrogans |
2.3.3.21 | H400A/H408A mutant | exists purely as a dimer in solution. Is inhibited by isoleucine with comparable IC50 and Ki values as the wild-type enzyme, the double mutations have very little effects on the binding (Km) of acetyl-CoA and the kcat. Binding of isoleucine with the mutant does not affect the dimeric state of the enzyme | Leptospira interrogans |
2.3.3.21 | I458A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, no inhibition by isoleucine, no binding of isoleucine | Leptospira interrogans |
2.3.3.21 | I458A | inhibition by isoleucine is severely decreased. Lower Kcat/Km as the wild-type | Leptospira interrogans |
2.3.3.21 | L451V | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values) | Leptospira interrogans |
2.3.3.21 | L451V | inhibition by isoleucine is severely decreased | Leptospira interrogans |
2.3.3.21 | P493A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values) | Leptospira interrogans |
2.3.3.21 | P493A | inhibition by isoleucine is greatly decreased | Leptospira interrogans |
2.3.3.21 | Q495A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values) | Leptospira interrogans |
2.3.3.21 | Q495A | exhibits significantly increased IC50 and Ki values for isoleucine | Leptospira interrogans |
2.3.3.21 | T464A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, minor effect on IC50 and Ki values for isoleucine | Leptospira interrogans |
2.3.3.21 | T464A | minor effect on the IC50 and Ki values for isoleucine | Leptospira interrogans |
2.3.3.21 | V468A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, slightly increased inhibition by isoleucine, and inhibition by leucine | Leptospira interrogans |
2.3.3.21 | V468A | inhibition by isoleucine is slightly decreased | Leptospira interrogans |
2.3.3.21 | Y430L | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values) | Leptospira interrogans |
2.3.3.21 | Y430L | inhibition by isoleucine is severely decreased | Leptospira interrogans |
2.3.3.21 | Y454A | changes hydrophobic residues at inhibitor binding site, minor effect on Km for acetyl-CoA but moderate decrease of kcat in the absence of inhibitor isoleucine, severely decreased inhibition by isoleucine (increase of IC50 and Ki values), slight inhibition by leucine | Leptospira interrogans |
2.3.3.21 | Y454A | inhibition by isoleucine is severely decreased. Lower Kcat/Km as the wild-type | Leptospira interrogans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.21 | isoleucine | end product feedback inhibition, competitive inhibition of the binding of pyruvate and acetyl-CoA; inhibits in a concentration-dependent manner. High selectivity of CMS for isoleucine over leucine is primarily dictated by the residues, Tyr430, Leu451, Tyr454, Ile458 and Val468, that form a hydrophobic pocket to accommodate the side chain of the inhibitor. Binding of isoleucine affects the binding of the substrate and coenzyme at the active site, possibly via conformational change of the dimer interface of the regulatory domain, leading to inhibition of the catalytic reaction, in a typical pattern of K-type inhibition | Leptospira interrogans | |
2.3.3.21 | leucine | inhibits only the mutants Y454A and V468A | Leptospira interrogans | |
2.3.3.21 | additional information | mutant I458A is not inhibited by isoleucine, leucine does not inhibit the wild-type and the mutants H400A/H408A, Y430L, L451V, I458A, D431A, T464A, P493A, and Q495A | Leptospira interrogans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.06 | - |
pyruvate | wild-type, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.06 | - |
pyruvate | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.094 | - |
pyruvate | wild-type, 1 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.094 | - |
pyruvate | wild-type, in the presence of 0.001 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.178 | - |
pyruvate | wild-type, 4 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.178 | - |
pyruvate | wild-type, in the presence of 0.004 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.626 | - |
acetyl-CoA | mutant T464A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.626 | - |
acetyl-CoA | T464A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.629 | - |
acetyl-CoA | D431A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.629 | - |
acetyl-CoA | mutant D431A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.712 | - |
acetyl-CoA | mutant Q495A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.712 | - |
acetyl-CoA | Q495A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.743 | - |
acetyl-CoA | mutant P493A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.743 | - |
acetyl-CoA | P493A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.803 | - |
acetyl-CoA | mutant Y454A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.803 | - |
acetyl-CoA | Y454A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.838 | - |
acetyl-CoA | I458A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.838 | - |
acetyl-CoA | mutant I458A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.097 | - |
acetyl-CoA | mutant Y430L, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.097 | - |
acetyl-CoA | Y430L 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.116 | - |
acetyl-CoA | mutant V468A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.116 | - |
acetyl-CoA | V468A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.118 | - |
acetyl-CoA | wild-type, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.118 | - |
acetyl-CoA | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.554 | - |
acetyl-CoA | H400A/H408A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.554 | - |
acetyl-CoA | mutant H400A/H408A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.711 | - |
acetyl-CoA | L451V 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.711 | - |
acetyl-CoA | mutant L451V, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.272 | - |
acetyl-CoA | wild-type, 20 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.272 | - |
acetyl-CoA | wild-type, in the presence of 0.02 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.246 | - |
acetyl-CoA | wild-type, 60 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.246 | - |
acetyl-CoA | wild-type, in the presence of 0.06 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.21 | Mn2+ | - |
Leptospira interrogans | |
2.3.3.21 | Zn2+ | bound, responsible for tetramer formation, removal or mutation of the binding sites by replacement of the histidine 400 and 408 by alanine leads to dimer formation | Leptospira interrogans | |
2.3.3.21 | Zn2+ | type II crystal structure contains two Zn2+ ions (which are located on the 2fold symmetry axis with an occupancy of 0.5) in the asymmetric unit. The type III crystal structure contains one Zn2+ ion in the asymmetric unit | Leptospira interrogans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 120000 | - |
wild-type full-length, Zn2+ removed by EDTA, gel filtration analysis | Leptospira interrogans |
2.3.3.21 | 128000 | - |
H400A/H408A mutant enzyme (preventing Zn2+-binding), gel filtration analysis | Leptospira interrogans |
2.3.3.21 | 310000 | - |
wild-type full-length, gel filtration analysis | Leptospira interrogans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate + H2O | Leptospira interrogans | - |
CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.21 | Leptospira interrogans | - |
- |
- |
2.3.3.21 | Leptospira interrogans | - |
leptospirosis pathogen | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.21 | - |
Leptospira interrogans |
2.3.3.21 | full-length and mutant CMS | Leptospira interrogans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate + H2O | - |
Leptospira interrogans | CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? | |
2.3.3.21 | acetyl-CoA + pyruvate + H2O | - |
Leptospira interrogans | CoA + (R)-citramalate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.21 | dimer | 2 * 120000, a tetramer of 2 head to head dimers of the smaller subunit LiCMSC are formed in the presence of Zn2+ | Leptospira interrogans |
2.3.3.21 | dimer | H400A/H408A mutant in solution, gel filtration | Leptospira interrogans |
2.3.3.21 | tetramer | purified wild-type in solution, gel filtration. Is presumably a tetramer due to the presence of Zn2+ co-purified with the enzyme from the expression system | Leptospira interrogans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.21 | citramalate synthase | - |
Leptospira interrogans |
2.3.3.21 | CMS | - |
Leptospira interrogans |
2.3.3.21 | LiCMS | - |
Leptospira interrogans |
2.3.3.21 | LiCMSC | regulatory domain of C-terminus of LiCMS | Leptospira interrogans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.6 | - |
acetyl-CoA | mutant Y454A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.6 | - |
acetyl-CoA | Y454A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.7 | - |
acetyl-CoA | I458A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.7 | - |
acetyl-CoA | mutant I458A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2 | - |
acetyl-CoA | D431A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2 | - |
acetyl-CoA | mutant D431A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.4 | - |
acetyl-CoA | L451V 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.4 | - |
acetyl-CoA | mutant L451V, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.4 | - |
acetyl-CoA | mutant T464A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.4 | - |
acetyl-CoA | T464A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.7 | - |
acetyl-CoA | mutant Q495A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.7 | - |
acetyl-CoA | Q495A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4 | - |
acetyl-CoA | mutant P493A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4 | - |
acetyl-CoA | P493A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.5 | - |
acetyl-CoA | mutant Y430L, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.5 | - |
acetyl-CoA | Y430L 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5 | - |
acetyl-CoA | mutant V468A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5 | - |
acetyl-CoA | V468A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.1 | - |
acetyl-CoA | H400A/H408A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.1 | - |
acetyl-CoA | mutant H400A/H408A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.1 | - |
pyruvate | wild-type, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.1 | - |
pyruvate | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.2 | - |
pyruvate | wild-type, 1 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.2 | - |
pyruvate | wild-type, in the presence of 0.001 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.3 | - |
pyruvate | wild-type, 4 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.3 | - |
pyruvate | wild-type, in the presence of 0.004 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 10.3 | - |
acetyl-CoA | wild-type, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 10.3 | - |
acetyl-CoA | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 10.6 | - |
acetyl-CoA | wild-type, 20 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 10.6 | - |
acetyl-CoA | wild-type, in the presence of 0.02 mM isoleucine 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 11.1 | - |
acetyl-CoA | wild-type, 60 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 11.1 | - |
acetyl-CoA | wild-type, in the presence of 0.06 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.009 | - |
isoleucine | mutant V468A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.009 | - |
isoleucine | V468A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.019 | - |
isoleucine | wild-type, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.019 | - |
isoleucine | wild-type, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.024 | - |
isoleucine | mutant T464A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.024 | - |
isoleucine | T464A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.043 | - |
isoleucine | H400A/H408A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.043 | - |
isoleucine | mutant H400A/H408A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.075 | - |
leucine | mutant V468A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.075 | - |
leucine | mutant V468A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.252 | - |
isoleucine | L451V 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.252 | - |
isoleucine | mutant L451V, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.382 | - |
isoleucine | D431A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.382 | - |
isoleucine | mutant D431A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.421 | - |
isoleucine | mutant Y454A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.421 | - |
isoleucine | Y454A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.581 | - |
isoleucine | mutant P493A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.581 | - |
isoleucine | P493A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.715 | - |
isoleucine | mutant Q495A, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 0.715 | - |
isoleucine | Q495A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.092 | - |
isoleucine | mutant Y430L, calculated from the Cheng-Prusoff equation | Leptospira interrogans | |
2.3.3.21 | 1.092 | - |
isoleucine | Y430L 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.302 | - |
leucine | mutant Y454A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.302 | - |
leucine | mutant Y454A, calculated from the Cheng-Prusoff equation | Leptospira interrogans |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.041 | - |
mutant V468A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.041 | - |
V468A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.086 | - |
wild-type, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.086 | - |
wild-type, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.153 | - |
H400A/H408A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.153 | - |
mutant H400A/H408A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.177 | - |
mutant T464A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.177 | - |
T464A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.343 | - |
mutant V468A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | leucine | |
2.3.3.21 | 0.343 | - |
mutant V468A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of leucine (approx. 0-20 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | leucine | |
2.3.3.21 | 0.839 | - |
L451V 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 0.839 | - |
mutant L451V, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 2.519 | - |
mutant Y454A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 2.519 | - |
Y454A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 2.815 | - |
D431A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 2.815 | - |
mutant D431A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 3.704 | - |
mutant P493A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 3.704 | - |
P493A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 4.733 | - |
mutant Q495A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 4.733 | - |
Q495A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 5.073 | - |
mutant Y430L, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 5.073 | - |
Y430L 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | isoleucine | |
2.3.3.21 | 7.786 | - |
mutant Y454A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | leucine | |
2.3.3.21 | 7.786 | - |
mutant Y454A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of leucine (approx. 0-20 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | leucine |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.3.21 | metabolism | first step in isoleucine biosynthesis | Leptospira interrogans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.74 | - |
acetyl-CoA | mutant Y454A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 0.8 | - |
acetyl-CoA | mutant I458A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.4 | - |
acetyl-CoA | L451V 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.4 | - |
acetyl-CoA | mutant L451V, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 1.5 | - |
pyruvate | wild-type, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.6 | - |
acetyl-CoA | wild-type, 60 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 2.6 | - |
acetyl-CoA | wild-type, in the presence of 0.06 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.2 | - |
acetyl-CoA | D431A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.2 | - |
acetyl-CoA | mutant D431A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.8 | - |
acetyl-CoA | mutant T464A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 3.8 | - |
acetyl-CoA | T464A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.1 | - |
acetyl-CoA | mutant Y430L, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.1 | - |
acetyl-CoA | Y430L 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.5 | - |
acetyl-CoA | mutant V468A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.5 | - |
acetyl-CoA | V468A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.6 | - |
acetyl-CoA | wild-type, 20 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 4.6 | - |
acetyl-CoA | wild-type, in the presence of 0.02 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.2 | - |
acetyl-CoA | mutant Q495A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.2 | - |
acetyl-CoA | Q495A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.2 | - |
pyruvate | wild-type, 4 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.4 | - |
acetyl-CoA | mutant P493A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.4 | - |
acetyl-CoA | P493A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.9 | - |
acetyl-CoA | H400A/H408A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 5.9 | - |
acetyl-CoA | mutant H400A/H408A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 7.4 | - |
acetyl-CoA | Y454A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 8 | - |
acetyl-CoA | I458A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.2 | - |
acetyl-CoA | wild-type, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.2 | - |
acetyl-CoA | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 9.8 | - |
pyruvate | wild-type, 1 microM isoleucine, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 52 | - |
pyruvate | wild-type, in the presence of 0.004 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 98 | - |
pyruvate | wild-type, in the presence of 0.001 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans | |
2.3.3.21 | 150 | - |
pyruvate | wild-type, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 | Leptospira interrogans |