Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ogawa, T.; Yoshimura, T.; Hemmi, H.
    Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution (2010), Biochem. Biophys. Res. Commun., 393, 16-20.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.81
-
 Methanosarcina mazei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.81 Mg2+ maximal activity around 5.0 mM, no activity with other divalent cations such as Ca2+, Mn2+, and Zn2+ Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.81 33000
-
 x * 33000, SDS-PAGE, calculated from sequence Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.81 farnesyl diphosphate + 2 isopentenyl diphosphate Methanosarcina mazei biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate Methanosarcina mazei biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.81 Methanosarcina mazei Q8PYS1 Gö1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.81
-
 Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.81 farnesyl diphosphate + 2 isopentenyl diphosphate biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.81 farnesyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 2 diphosphate (2E,6E,10E,14E)-geranylfarnesyl diphosphate is the main product, a considerable amount of geranylgeranyl diphosphate as an intermediate is also observed ?
2.5.1.81 geranyl diphosphate + 3 isopentenyl diphosphate slight activity Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + 3 diphosphate
-
 ?
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate Methanosarcina mazei (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
2.5.1.81 additional information dimethylally diphosphate is not accepted as substrate Methanosarcina mazei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.81 ? x * 33000, SDS-PAGE, calculated from sequence Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
2.5.1.81 MM_0789
-
 Methanosarcina mazei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.81 40
-
-
 Methanosarcina mazei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.81 7
-
-
 Methanosarcina mazei