EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.48 | recombinant expression of the viral His18-tagged enzyme components in Sf21AE and TN5 insect cells, establishing a single-round transcription system | influenza A virus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.48 | additional information | - |
additional information | kinetic analysis of the recombinant enzyme complex | influenza A virus | |
2.7.7.48 | 0.0027 | - |
CTP | pH 8.0, 25°C, HCV JFH1a de novo transcription | influenza A virus | |
2.7.7.48 | 0.0047 | - |
ATP | pH 8.0, 25°C, HCV JFH1a de novo transcription | influenza A virus | |
2.7.7.48 | 0.0075 | - |
UTP | pH 8.0, 25°C, HCV JFH1a de novo transcription | influenza A virus | |
2.7.7.48 | 0.0107 | - |
GTP | pH 8.0, 25°C, Influenza ApG-primed transcription | influenza A virus | |
2.7.7.48 | 0.022 | - |
UTP | pH 8.0, 25°C, Influenza ApG-primed transcription | influenza A virus | |
2.7.7.48 | 0.0289 | - |
CTP | pH 8.0, 25°C, Influenza ApG-primed transcription | influenza A virus | |
2.7.7.48 | 0.0332 | - |
ATP | pH 8.0, 25°C, Influenza ApG-primed transcription | influenza A virus | |
2.7.7.48 | 0.087 | - |
GTP | pH 8.0, 25°C, HCV JFH1a de novo transcription | influenza A virus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | Mg2+ | activates optimally at 8 mM, recombinant viral enzyme purified from insect cells | influenza A virus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | influenza A virus | - |
diphosphate + RNAn+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.48 | influenza A virus | - |
from insect cells | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.48 | recombinant viral His18-tagged enzyme components from Sf21AE and TN5 insect cells | influenza A virus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | ATP + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | CTP + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | GTP + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | additional information | the RdRp catalyzes all the biochemical reactions of influenza virus transcription and replication in vitro, dinucleotide ApG and globin mRNA-primed transcription, de novo initiation/replication, and polyadenylation | influenza A virus | ? | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | in vitro transcription using the model RNA template, v84 | influenza A virus | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | UTP + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.48 | RDRP | - |
influenza A virus |
2.7.7.48 | RNA polymerase | - |
influenza A virus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 25 | - |
recombinant viral enzyme purified from insect cells | influenza A virus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 4 | 42 | 28.7% at 4°C and 13.4% at 42°C of the activity at 25°C, no activity at 0°C | influenza A virus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 7 | 8.5 | wild-type enzyme, broad optimum | influenza A virus |
2.7.7.48 | 8 | - |
recombinant viral enzyme purified from insect cells | influenza A virus |