EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.39 | expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.6.1.39 | complexed with N-(5'-phosphopyridoxyl)-L-glutamate, vapor diffusion method, using 16% (w/v) PEG3350, 100 mM HEPES, pH 7.0, and 200 mM calcium acetate, at 20°C | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.39 | R23A | the mutation causes 4.1fold increase in the Km value for L-2-aminoadipate, the Km value for L-Glu increases 546fold, the Km value for L-Leu is not greatly affected | Thermus thermophilus |
2.6.1.39 | R23Q | the mutation causes 13.7fold increase in the Km value for L-2-aminoadipate, the Km value for L-Glu increases 134fold, the Km value for L-Leu is not greatly affected | Thermus thermophilus |
2.6.1.39 | S20E | the mutant shows decreased kcat values compared to the wild type enzyme | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.39 | 0.048 | - |
2-oxoisocaproate | mutant enzyme R23A, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.05 | - |
2-oxoisocaproate | mutant enzyme S20E, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.091 | - |
2-oxoisocaproate | mutant enzyme R23Q, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.2 | - |
L-leucine | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.27 | - |
2-oxoglutarate | wild type enzyme, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.3 | - |
2-oxoglutarate | wild type enzyme, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.39 | - |
L-leucine | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.4 | - |
2-oxoglutarate | mutant enzyme S20E, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.41 | - |
2-oxoglutarate | mutant enzyme S20E, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.42 | - |
2-oxoglutarate | mutant enzyme R23Q, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.43 | - |
2-oxoisocaproate | wild type enzyme, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.46 | - |
L-glutamate | wild type enzyme, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.54 | - |
2-oxoglutarate | mutant enzyme R23A, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.55 | - |
2-oxoglutarate | mutant enzyme R23Q, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.61 | - |
2-oxoglutarate | mutant enzyme R23A, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.81 | - |
L-2-aminoadipate | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.88 | - |
L-leucine | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.95 | - |
L-leucine | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 3.3 | - |
L-2-aminoadipate | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 7 | - |
L-2-aminoadipate | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 11 | - |
L-2-aminoadipate | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 62 | - |
L-glutamate | mutant enzyme R23Q, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 82 | - |
L-glutamate | mutant enzyme S20E, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 250 | - |
L-glutamate | mutant enzyme R23A, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.39 | Thermus thermophilus | Q72LL6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.39 | ammonium sulfate precipitation and Superdex 200 gel filtration | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.39 | 2-oxoisocaproate + L-glutamate | - |
Thermus thermophilus | ? | - |
? | |
2.6.1.39 | L-2-aminoadipate + 2-oxoglutarate | - |
Thermus thermophilus | 2-oxoadipate + L-glutamate | - |
? | |
2.6.1.39 | L-leucine + 2-oxoglutarate | - |
Thermus thermophilus | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
2.6.1.39 | additional information | negligible activity is observed for L-Asp | Thermus thermophilus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.39 | AAA-AT | - |
Thermus thermophilus |
2.6.1.39 | alpha-aminoadipate aminotransferase | - |
Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.39 | 0.009 | - |
L-leucine | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.011 | - |
L-2-aminoadipate | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.023 | - |
L-leucine | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.04 | - |
L-2-aminoadipate | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.05 | - |
L-leucine | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.083 | - |
L-2-aminoadipate | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.23 | - |
L-glutamate | mutant enzyme R23A, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.5 | - |
L-glutamate | mutant enzyme R23Q, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 1.2 | - |
L-glutamate | mutant enzyme S20E, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 5.1 | - |
L-leucine | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 6.7 | - |
L-2-aminoadipate | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 16 | - |
L-glutamate | wild type enzyme, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.39 | pyridoxal 5'-phosphate | - |
Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.39 | 0.0009 | - |
L-glutamate | mutant enzyme R23A, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.0033 | - |
L-2-aminoadipate | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.0057 | - |
L-2-aminoadipate | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.0075 | - |
L-2-aminoadipate | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.0081 | - |
L-glutamate | mutant enzyme R23Q, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.014 | - |
L-glutamate | mutant enzyme S20E, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.015 | - |
2-oxoglutarate | mutant enzyme R23A, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.021 | - |
2-oxoglutarate | mutant enzyme R23A, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.023 | - |
L-leucine | mutant enzyme R23A, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.057 | - |
L-leucine | mutant enzyme R23Q, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.06 | - |
2-oxoglutarate | mutant enzyme S20E, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.091 | - |
2-oxoglutarate | mutant enzyme R23Q, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.097 | - |
2-oxoglutarate | mutant enzyme S20E, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.12 | - |
L-leucine | mutant enzyme S20E, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 0.2 | - |
2-oxoglutarate | mutant enzyme R23Q, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 4.8 | - |
2-oxoisocaproate | mutant enzyme R23A, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 5.4 | - |
L-leucine | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 5.5 | - |
2-oxoisocaproate | mutant enzyme R23Q, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 8.2 | - |
L-2-aminoadipate | wild type enzyme, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 19 | - |
2-oxoglutarate | wild type enzyme, using L-leucine as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 22 | - |
2-oxoglutarate | wild type enzyme, using L-2-aminoadipate as cosubstrate, in 50 mM HEPES-NaOH buffer, pH 8.0, 50 mM KCl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 24 | - |
2-oxoisocaproate | mutant enzyme S20E, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 34 | - |
2-oxoisocaproate | wild type enzyme, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus | |
2.6.1.39 | 37 | - |
L-glutamate | wild type enzyme, using 2-oxoisocaproate as cosubstrate, in 50 mM HEPESNaOH buffer, pH 8.0, containing 50 mM NH4Cl, 0.15 mM pyridoxal 5'-phosphate, at 45°C | Thermus thermophilus |