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Literature summary extracted from
- Different catalytic properties of two highly homologous triosephosphate isomerase monomers (2009), Biochem. Biophys. Res. Commun., 382, 626-630.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | expression of the monomeric enzyme in Escherichia coli strain BL21 (DE3) | Trypanosoma cruzi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | purified recombinant monomeric enzyme by sitting drop method, 0.001 ml of protein solutioncontaining 25 mg/ml protein in 20 mM Tris-HCl , pH 7.4, and 1.0 mM EDTA is mixed with 0.001 ml of reservoir solution containing 100 mM HEPES, pH 7.5, 10% PEG 6000, and 5% MPD, X-ray diffraction structure determination and analysis | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-Glyceraldehyde 3-phosphate | Trypanosoma cruzi | - |
Glycerone phosphate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Trypanosoma cruzi | P52270 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | recombinant monomeric enzyme from Escherichia coli strain BL21 (DE3) | Trypanosoma cruzi |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | unfolding/refolding reactions of monomeric enzyme evaluated using the two-state model | Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-Glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | Glycerone phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | dimer | active enzyme form | Trypanosoma cruzi |
| 5.3.1.1 | More | structure determination and analysis of the monomeric enzyme, overview | Trypanosoma cruzi |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | Triosephosphate isomerase | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 25 | - |
assay at | Trypanosoma cruzi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 7.4 | - |
assay at | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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