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Literature summary extracted from

  • Leonard, E.; Yan, Y.; Chemler, J.; Matern, U.; Martens, S.; Koffas, M.
    Characterization of dihydroflavonol 4-reductases for recombinant plant pigment biosynthesis applications (2008), Biocatal. Biotransform., 26, 243-251.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Rosa hybrid cultivar
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Arabidopsis thaliana
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Anthurium andraeanum
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Lilium sp.
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Fragaria x ananassa
1.1.1.219 into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Ipomoea nil

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Anthurium andraeanum Q84L22
-
-
1.1.1.219 Arabidopsis thaliana P51102
-
-
1.1.1.219 Fragaria x ananassa O22617
-
-
1.1.1.219 Ipomoea nil O24607
-
-
1.1.1.219 Lilium sp. Q6UAQ7 hybrid, cv. Star Gazer
-
1.1.1.219 Rosa hybrid cultivar Q41158 cv. minirose
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower
-
 Rosa hybrid cultivar
-
1.1.1.219 flower
-
 Lilium sp.
-
1.1.1.219 fruit
-
 Fragaria x ananassa
-
1.1.1.219 additional information spathe Anthurium andraeanum
-
1.1.1.219 petal
-
 Rosa hybrid cultivar
-
1.1.1.219 petal
-
 Lilium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.219 0.00002
-
 with dihydrokaempferol as substrate Arabidopsis thaliana
1.1.1.219 0.0003
-
 with dihydrokaempferol as substrate Ipomoea nil
1.1.1.219 0.0003
-
 with naringenin as substrate Ipomoea nil
1.1.1.219 0.0004
-
 with dihydrokaempferol as substrate Rosa hybrid cultivar
1.1.1.219 0.0004
-
 with naringenin as substrate Rosa hybrid cultivar
1.1.1.219 0.0005
-
 with naringenin as substrate Fragaria x ananassa
1.1.1.219 0.0006
-
 with eriodictyol as substrate Arabidopsis thaliana
1.1.1.219 0.0006
-
 with eriodictyol as substrate Lilium sp.
1.1.1.219 0.0006
-
 with eriodictyol as substrate Fragaria x ananassa
1.1.1.219 0.0006
-
 with eriodictyol as substrate Ipomoea nil
1.1.1.219 0.0007
-
 with eriodictyol as substrate Rosa hybrid cultivar
1.1.1.219 0.0007
-
 with naringenin as substrate Anthurium andraeanum
1.1.1.219 0.0008
-
 with eriodictyol as substrate Anthurium andraeanum
1.1.1.219 0.001
-
 with dihydromyricetin as substrate Arabidopsis thaliana
1.1.1.219 0.001
-
 with dihydroquercitin as substrate Rosa hybrid cultivar
1.1.1.219 0.0012
-
 with dihydromyricetin as substrate Lilium sp.
1.1.1.219 0.0012
-
 with dihydroquercitin as substrate Fragaria x ananassa
1.1.1.219 0.0013
-
 with dihydromyricetin as substrate Rosa hybrid cultivar
1.1.1.219 0.0013
-
 with dihydroquercitin as substrate Arabidopsis thaliana
1.1.1.219 0.0013
-
 with dihydroquercitin as substrate Lilium sp.
1.1.1.219 0.0013
-
 with dihydroquercitin as substrate Ipomoea nil
1.1.1.219 0.0014
-
 with dihydromyricetin as substrate Anthurium andraeanum
1.1.1.219 0.0014
-
 with dihydromyricetin as substrate Fragaria x ananassa
1.1.1.219 0.0014
-
 with dihydroquercitin as substrate Anthurium andraeanum
1.1.1.219 0.0015
-
 with dihydromyricetin as substrate Ipomoea nil
1.1.1.219 0.014
-
 with dihydrokaempferol as substrate Fragaria x ananassa
1.1.1.219 0.021
-
 with dihydrokaempferol as substrate Anthurium andraeanum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 7-hydroxyflavanone + NADPH + H+
-
 Anthurium andraeanum 2,4-cis-7-hydroxyflavan-4-ol + 2,4-trans-7-hydroxyflavan-4-ol + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Rosa hybrid cultivar leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Arabidopsis thaliana leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Fragaria x ananassa leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Ipomoea nil leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Anthurium andraeanum leucopelargonidin + NADP+ highest pelargonidin concentration derived from the E-color culture harboring the Anthurium andraeanum DFR ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Rosa hybrid cultivar leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Arabidopsis thaliana leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Anthurium andraeanum leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Lilium sp. leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Fragaria x ananassa leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Ipomoea nil leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Rosa hybrid cultivar leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Arabidopsis thaliana leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Anthurium andraeanum leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Lilium sp. leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Fragaria x ananassa leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Ipomoea nil leucocyanidin + NADP+
-
 ?
1.1.1.219 eriodictyol + NADPH + H+
-
 Rosa hybrid cultivar luteoforol + NADP+
-
 ?
1.1.1.219 eriodictyol + NADPH + H+
-
 Anthurium andraeanum luteoforol + NADP+
-
 ?
1.1.1.219 eriodictyol + NADPH + H+
-
 Lilium sp. luteoforol + NADP+
-
 ?
1.1.1.219 eriodictyol + NADPH + H+
-
 Fragaria x ananassa luteoforol + NADP+
-
 ?
1.1.1.219 eriodictyol + NADPH + H+
-
 Ipomoea nil luteoforol + NADP+
-
 ?
1.1.1.219 additional information can not reduce hesperetin or 5,7-dimethoxyflavanone Anthurium andraeanum ?
-
 ?
1.1.1.219 additional information does not catalyze dihydrokaempferol and naringenin Lilium sp. ?
-
 ?
1.1.1.219 additional information does not catalyze naringenin Arabidopsis thaliana ?
-
 ?
1.1.1.219 naringenin + NADPH + H+
-
 Rosa hybrid cultivar apiferol + NADP+
-
 ?
1.1.1.219 naringenin + NADPH + H+
-
 Anthurium andraeanum apiferol + NADP+
-
 ?
1.1.1.219 naringenin + NADPH + H+
-
 Fragaria x ananassa apiferol + NADP+
-
 ?
1.1.1.219 naringenin + NADPH + H+
-
 Ipomoea nil apiferol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Rosa hybrid cultivar
1.1.1.219 DFR
-
 Arabidopsis thaliana
1.1.1.219 DFR
-
 Anthurium andraeanum
1.1.1.219 DFR
-
 Lilium sp.
1.1.1.219 DFR
-
 Fragaria x ananassa
1.1.1.219 DFR
-
 Ipomoea nil
1.1.1.219 dihydroflavonol 4-reductase
-
 Rosa hybrid cultivar
1.1.1.219 dihydroflavonol 4-reductase
-
 Arabidopsis thaliana
1.1.1.219 dihydroflavonol 4-reductase
-
 Anthurium andraeanum
1.1.1.219 dihydroflavonol 4-reductase
-
 Lilium sp.
1.1.1.219 dihydroflavonol 4-reductase
-
 Fragaria x ananassa
1.1.1.219 dihydroflavonol 4-reductase
-
 Ipomoea nil

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.219 25 30 maximum DFR activities achieved towards dihydroflavonols and flavanones Anthurium andraeanum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 6 7 maximum DFR activities achieved towards dihydroflavonols and flavanones Anthurium andraeanum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Rosa hybrid cultivar
1.1.1.219 NADPH
-
 Arabidopsis thaliana
1.1.1.219 NADPH
-
 Anthurium andraeanum
1.1.1.219 NADPH
-
 Lilium sp.
1.1.1.219 NADPH
-
 Fragaria x ananassa
1.1.1.219 NADPH
-
 Ipomoea nil

General Information

EC Number General Information Comment Organism
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Rosa hybrid cultivar
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Arabidopsis thaliana
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Anthurium andraeanum
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Lilium sp.
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Fragaria x ananassa
1.1.1.219 physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Ipomoea nil