EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | Hg2+ | MCM activity is inhibited completely by the addition of 3 mM Hg2+ | Euglena gracilis | |
5.4.99.2 | additional information | Na+, K+, Ca2+, Fe2+, Mg2+, and Co2+ (all at 3 mM) and 3 mM EDTA do not cause inhibition of the MCM activity | Euglena gracilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.2 | 0.0279 | - |
(R)-2-methylmalonyl-CoA | in 100 mM potassium phosphate buffer (pH 7.5), at 37°C | Euglena gracilis | |
5.4.99.2 | 0.3474 | - |
succinyl-CoA | in 100 mM potassium phosphate buffer (pH 7.5), at 37°C | Euglena gracilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.4.99.2 | mitochondrion | - |
Euglena gracilis | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.2 | 75000 | - |
2 * 75000, SDS-PAGE | Euglena gracilis |
5.4.99.2 | 78250 | - |
2 * 78250, calculated from amino acid sequence | Euglena gracilis |
5.4.99.2 | 149000 | - |
gel filtration | Euglena gracilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.2 | Euglena gracilis | B7XBM0 | - |
- |
5.4.99.2 | Euglena gracilis Z | B7XBM0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.2 | ammonium sulfate precipitation, TSKgel phenyl-Toyopearl column chromatography, TSKgel QAE-Toyopearl column chromatography, Bio-Scale CHT2-1 column chromatography, UNO Q-1 column chromatography, and Superdex 200 gel filtration | Euglena gracilis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.99.2 | 0.06 | - |
crude homogenate, at 40°C | Euglena gracilis |
5.4.99.2 | 21 | - |
after 350fold purification, at 40°C | Euglena gracilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Euglena gracilis | succinyl-CoA | - |
r | |
5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Euglena gracilis Z | succinyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.2 | homodimer | 2 * 75000, SDS-PAGE | Euglena gracilis |
5.4.99.2 | homodimer | 2 * 78250, calculated from amino acid sequence | Euglena gracilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.2 | MCM | - |
Euglena gracilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.2 | 35 | - |
- |
Euglena gracilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.2 | 25 | 50 | the enzyme, when incubated at various temperatures for 10 min at pH 7.0, is stable up to 25°C, and complete loss of activity is observed at 50°C | Euglena gracilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.2 | 7 | - |
- |
Euglena gracilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | 5'-deoxyadenosylcobalamin | the apoenzyme is converted to a holoenzyme by incubation for 4 h at 4°C with 0.01 mM 5'-deoxyadenosylcobalamin, the purified enzyme contains one mole of prosthetic 5'-deoxyadenosylcobalamin per mole of subunit | Euglena gracilis | |
5.4.99.2 | adenosylcobalamin | the apparent Km value of the apoenzyme is 22.7 nM | Euglena gracilis |