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Literature summary extracted from

  • Kumar, S.; Parvathi, A.; Hernandez, R.L.; Cadle, K.M.; Varela, M.F.
    Identification of a novel UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Vibrio fischeri that confers high fosfomycin resistance in Escherichia coli (2009), Arch. Microbiol., 191, 425-429.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.7 drug development MurA will be a useful target to identify potential inhibitors of fosfomycin resistance in pharmacological studies Aliivibrio fischeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 overexpression in Escherichia coli with a 6-His tag Aliivibrio fischeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.7 44700
-
 calculated from the 422 amino acids, confirmed by SDS-PAGE Aliivibrio fischeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Aliivibrio fischeri
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Aliivibrio fischeri B6DVJ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 by metal affinity chromatography with a nickel column Aliivibrio fischeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Aliivibrio fischeri phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 MurA
-
 Aliivibrio fischeri
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Aliivibrio fischeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.7 Aliivibrio fischeri estimated
-
 5

General Information

EC Number General Information Comment Organism
2.5.1.7 physiological function key enzyme is involved in bacterial cell wall peptidoglycan synthesis and a target for the antimicrobial agent fosfomycin, a structural analog of the MurA substrate phosphoenol pyruvate Aliivibrio fischeri