EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.18 | medicine | GSTs catalyzing inactivation of anticancer drugs may find clinical use in protecting sensitive normal tissues to toxic side effects in treated patients, and as selectable markers in gene therapy | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.18 | recombinant expression of the His6-tagged isozyme GST T1-1 in Escherichia coli | Mus musculus |
2.5.1.18 | recombinant expression of the His6-tagged wild-type and mutant isozymes GST T1-1 in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.18 | C14S/T226I/W234R | construction of chimeric mutant F2:1215 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F2:1215 shows highly increased activity compared to the human enzyme and increased activity compared to the murine enzyme, overview | Homo sapiens |
2.5.1.18 | additional information | construction of chimeric mutants F2:1215 and F5:1474 comprising sequences from the murine isozymes GST-T1-1, as well as from the isozymes GST-T1-1 and GST-T2 of Homo sapiens, the mutant F2:1215 and mutant F5:1474 show increased activity compared to the human and to the murine enzyme, overview | Mus musculus |
2.5.1.18 | W234R | construction of chimeric mutant F5:1474 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F5:1474 shows increased activity compared to the human enzyme and to the murine enzyme, overview | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | additional information | - |
additional information | steady-state kinetics of recombinant His6-tagged isozyme GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea | Mus musculus | |
2.5.1.18 | additional information | - |
additional information | steady-state kinetics of recombinant His6-tagged wild-type and mutant isozymes GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea | Homo sapiens | |
2.5.1.18 | 0.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
2.5.1.18 | 0.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens | |
2.5.1.18 | 0.8 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
2.5.1.18 | 1 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.18 | additional information | Homo sapiens | the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 | ? | - |
? | |
2.5.1.18 | additional information | Mus musculus | the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.18 | Homo sapiens | - |
isozyme GST T1-1 | - |
2.5.1.18 | Mus musculus | - |
isozyme GST T1-1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.18 | 1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Mus musculus | ? | - |
? | |
2.5.1.18 | 1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Homo sapiens | ? | - |
? | |
2.5.1.18 | 1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione | - |
Mus musculus | ? | - |
? | |
2.5.1.18 | 1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione | - |
Homo sapiens | ? | - |
? | |
2.5.1.18 | 4-nitrophenethyl bromide + glutathione | - |
Mus musculus | ? | - |
? | |
2.5.1.18 | 4-nitrophenethyl bromide + glutathione | - |
Homo sapiens | ? | - |
? | |
2.5.1.18 | additional information | the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 | Homo sapiens | ? | - |
? | |
2.5.1.18 | additional information | the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 | Mus musculus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.18 | GST T1-1 | - |
Mus musculus |
2.5.1.18 | GST T1-1 | - |
Homo sapiens |
2.5.1.18 | theta-class glutathione transferase | - |
Mus musculus |
2.5.1.18 | theta-class glutathione transferase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 37 | - |
assay at | Mus musculus |
2.5.1.18 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.035 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens | |
2.5.1.18 | 2.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
2.5.1.18 | 3.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
2.5.1.18 | 3.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 7.4 | - |
assay at | Mus musculus |
2.5.1.18 | 7.4 | - |
assay at | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.036 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens | |
2.5.1.18 | 2.9 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
2.5.1.18 | 6.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
2.5.1.18 | 6.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens |