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Literature summary extracted from

  • Jeya, M.; Joo, A.R.; Lee, K.M.; Tiwari, M.K.; Lee, K.M.; Kim, S.H.; Lee, J.K.
    Characterization of beta-glucosidase from a strain of Penicillium purpurogenum KJS506 (2010), Appl. Microbiol. Biotechnol., 86, 1473-1484.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucose competitive inhibition Talaromyces purpureogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 5.1
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM sodium acetate buffer, pH 5.0, at 65°C Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 89620
-
 calculated from amino acid sequence Talaromyces purpureogenus
3.2.1.21 110000
-
 SDS-PAGE Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Talaromyces purpureogenus C9E9M9
-
-
3.2.1.21 Talaromyces purpureogenus KJS506 C9E9M9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein
-
 Talaromyces purpureogenus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 DEAE Sepharose column chromatography, MonoQ column chromatography, and hydroxyapatite column chromatography Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-mannopyranoside + H2O 0.7% activity compared to 4-nitrophenyl-beta-D-mannopyranoside Talaromyces purpureogenus 2-nitrophenol + D-mannopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O 11.6% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O 8.4% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O 8.4% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus KJS506 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Talaromyces purpureogenus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Talaromyces purpureogenus KJS506 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 Avicel + H2O 0.9% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus beta-D-glucose + ?
-
 ?
3.2.1.21 Avicel + H2O 0.9% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus KJS506 beta-D-glucose + ?
-
 ?
3.2.1.21 cellobiose + H2O 49.4% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O 49.4% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus KJS506 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O 7.8% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O 11% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O 12.7% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Talaromyces purpureogenus 3 D-glucose
-
 ?
3.2.1.21 additional information no activity towards 4-nitrophenyl-alpha-D-galactopyranoside, carboxymethylcellulose, and xylan (2% w/v) Talaromyces purpureogenus ?
-
 ?
3.2.1.21 additional information no activity towards 4-nitrophenyl-alpha-D-galactopyranoside, carboxymethylcellulose, and xylan (2% w/v) Talaromyces purpureogenus KJS506 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 110000, SDS-PAGE Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bgl
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65
-
-
 Talaromyces purpureogenus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 50 70
-
 Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40 80 the purified enzyme shows a half-life of 10 h at 65°C, about 20% activity at 40°C, about 60% activity at 50°C, about 90% activity at 60°C, about 80% activity at 70°C, less than 20% activity at 80°C Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Talaromyces purpureogenus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4 6
-
 Talaromyces purpureogenus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 3 7 no activity at pH 3.0 and 7.0, about 20% activity at pH 4.0, about 40% activity at pH 6.0 Talaromyces purpureogenus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 21.5
-
 D-glucose
-
 Talaromyces purpureogenus