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Literature summary extracted from
- A periplasmic, pyridoxal-5-phosphate-dependent amino acid racemase in Pseudomonas taetrolens (2009), Appl. Microbiol. Biotechnol., 83, 1045-1054.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.10 | expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas taetrolens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.1.1.10 | periplasm | - |
Pseudomonas taetrolens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 41500 | - |
calculated from cDNA | Pseudomonas taetrolens |
| 5.1.1.10 | 84000 | - |
gel filtration | Pseudomonas taetrolens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.10 | Pseudomonas taetrolens | I0J1I6 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 5.1.1.10 | proteolytic modification | N-terminal signal sequence is cleaved off | Pseudomonas taetrolens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.10 | using Ni-NTA chromatography | Pseudomonas taetrolens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 1120 | - |
recombinant protein | Pseudomonas taetrolens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.10 | D-Ala | 12% activity, compared to D-Lys | Pseudomonas taetrolens | L-Ala | - |
? |  |
| 5.1.1.10 | D-Arg | 95% activity, compared to D-Lys | Pseudomonas taetrolens | L-Arg | - |
? | |
| 5.1.1.10 | D-ethionine | 11% activity, compared to D-Lys | Pseudomonas taetrolens | L-ethionine | - |
? | |
| 5.1.1.10 | D-homoarginine | 6.2% activity, compared to D-Lys | Pseudomonas taetrolens | L-homoarginine | - |
? | |
| 5.1.1.10 | D-Lys | 100% activity | Pseudomonas taetrolens | L-Lys | - |
? | |
| 5.1.1.10 | D-Met | 12% activity, compared to D-Lys | Pseudomonas taetrolens | L-Met | - |
? | |
| 5.1.1.10 | D-norvaline | 6.5% activity, compared to D-Lys | Pseudomonas taetrolens | L-norvaline | - |
? | |
| 5.1.1.10 | D-ornithine | 42% activity, compared to D-Lys | Pseudomonas taetrolens | L-ornithine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.10 | dimer | 2 * 41500 Da | Pseudomonas taetrolens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.1.10 | amino acid racemase | - |
Pseudomonas taetrolens |
| 5.1.1.10 | ArgR | - |
Pseudomonas taetrolens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 37 | - |
assay at | Pseudomonas taetrolens |
| 5.1.1.10 | 65 | - |
maximum activity | Pseudomonas taetrolens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 8 | - |
optimal activity | Pseudomonas taetrolens |
| 5.1.1.10 | 10 | - |
assay at | Pseudomonas taetrolens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.10 | pyridoxal 5'-phosphate | - |
Pseudomonas taetrolens | |
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Release 2023.1 (January 2023)
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