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Literature summary extracted from

  • Yu, Y.; Ramsay, J.A.; Ramsay, B.A.
    Use of allylthiourea to produce soluble methane monooxygenase in the presence of copper (2009), Appl. Microbiol. Biotechnol., 82, 333-339.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.25 biotechnology method by which sMMO can be produced by strain OB3b while growing on methanol in copper-containing medium Methylosinus trichosporium OB3b

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.25 Allylthiourea pMMO inhibitor and copper chelator. Without allylthiourea strain OB3b quickly loses sMMO activity whenever it is grown on NMS medium with methane as the sole carbon and energy source. No loss of sMMO activity when the growth substrate is switched from methane to methanol when allylthiourea is added to growth medium containing copper Methylosinus trichosporium OB3b
1.14.13.25 Cu2+ loss of sMMO activity with copper addition to a culture growing on methanol. Recovery of sMMO activity by addition of allylthiourea Methylosinus trichosporium OB3b

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium OB3b
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
Methylosinus trichosporium OB3b methanol + NAD(P)+ + H2O
-
?
1.14.13.25 naphthalene + NAD(P)H + O2
-
Methylosinus trichosporium OB3b alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 sMMO
-
Methylosinus trichosporium OB3b
1.14.13.25 soluble methane monooxygenase
-
Methylosinus trichosporium OB3b

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylosinus trichosporium OB3b
1.14.13.25 NADPH
-
Methylosinus trichosporium OB3b