EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.99 | CaCl2 | 69% residual activity at 10 mM | Rhodotorula sp. | |
2.4.1.99 | Citric acid | 65% residual activity at 10 mM | Rhodotorula sp. | |
2.4.1.99 | KCl | 40% residual activity at 10 mM | Rhodotorula sp. | |
2.4.1.99 | MnCl2 | 99% residual activity at 10 mM | Rhodotorula sp. | |
2.4.1.99 | NaCl | 74% residual activity at 10 mM | Rhodotorula sp. | |
2.4.1.99 | ZnSO4 | 68% residual activity at 10 mM | Rhodotorula sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.99 | extracellular | - |
Rhodotorula sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.99 | CuSO4 | 121% activity at 10 mM | Rhodotorula sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 77000 | - |
2 * 77000, SDS-PAGE | Rhodotorula sp. |
2.4.1.99 | 170000 | - |
gel filtration | Rhodotorula sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.99 | sucrose + sucrose | Rhodotorula sp. | - |
D-glucose + 1-kestose | - |
? | |
2.4.1.99 | sucrose + sucrose | Rhodotorula sp. LEB-V10 | - |
D-glucose + 1-kestose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.99 | Rhodotorula sp. | - |
- |
- |
2.4.1.99 | Rhodotorula sp. LEB-V10 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.99 | Q sepharose column chromatography | Rhodotorula sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 0.5 | - |
crude extract | Rhodotorula sp. |
2.4.1.99 | 75.4 | - |
after 150.8fold purification | Rhodotorula sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.99 | sucrose + sucrose | - |
Rhodotorula sp. | D-glucose + 1-kestose | - |
? | |
2.4.1.99 | sucrose + sucrose | - |
Rhodotorula sp. LEB-V10 | D-glucose + 1-kestose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.99 | homodimer | 2 * 77000, SDS-PAGE | Rhodotorula sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.99 | fructooligosaccharide-producing extracellular enzyme | - |
Rhodotorula sp. |
2.4.1.99 | fructosyl-transferase | - |
Rhodotorula sp. |
2.4.1.99 | FTA | - |
Rhodotorula sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 65 | 70 | - |
Rhodotorula sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 66 | - |
very stable at temperatures below 66°C, the half-life of the enzyme decreases very quickly at temperatures above 66°C | Rhodotorula sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.99 | 5 | - |
around pH 5.0 | Rhodotorula sp. |