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Literature summary extracted from

  • Han, J.; Lu, Q.; Zhou, L.; Liu, H.; Xiang, H.
    Identification of the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A reductase among multiple FabG paralogs in Haloarcula hispanica and reconstruction of the PHA biosynthetic pathway in Haloferax volcanii (2009), Appl. Environ. Microbiol., 75, 6168-6175.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 gene fabG1, DNA sequence determination and analysis, expression in the fabG1-deficient mutant, phylogenetic tree, sequence comparison of FabG1 to FabG6 Haloarcula hispanica
1.1.1.100 heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with fabG1 in Haloferax volcanii Haloarcula hispanica
1.1.1.100 heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with Haloarcula hispanica fabG1 reconstructs the polyhydroxyalkanoate biosynthetic pathway Haloferax volcanii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.36 additional information disruption or knockout of fabG1 abolishes HA synthesis, and complementation of the DELTAfabG1 mutant with the fabG1 gene restores both PHA synthesis capability and the NADPH-dependent acetoacetyl-CoA reductase activity. Heterologous coexpression of the PHA synthase genes, phaEC together with fabG1, but not its five paralogs, reconstructs the PHA biosynthetic pathway in Haloferax volcanii, a PHA-defective haloarchaeon Haloarcula hispanica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Haloarcula hispanica FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Haloarcula hispanica DSM 4426 FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Haloarcula hispanica G0HY85 or ATCC 33960, gene fabG1
-
1.1.1.36 Haloarcula hispanica DSM 4426 G0HY85 or ATCC 33960, gene fabG1
-
1.1.1.100 Haloarcula hispanica
-
-
-
1.1.1.100 Haloarcula hispanica AS2049
-
-
-
1.1.1.100 Haloferax volcanii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Haloarcula hispanica (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase Haloarcula hispanica (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Haloarcula hispanica DSM 4426 (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase Haloarcula hispanica DSM 4426 (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.36 More FabG belongs to the short-chain dehydrogenase/reductase superfamily, whose members are homologous in sequence and have several conserved motifs Haloarcula hispanica
1.1.1.36 PHA-specific acetoacetyl-CoA reductase
-
 Haloarcula hispanica
1.1.1.36 polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase
-
 Haloarcula hispanica
1.1.1.100 beta-ketoacyl-ACP reductase
-
 Haloferax volcanii
1.1.1.100 beta-ketoacyl-ACP reductase
-
 Haloarcula hispanica
1.1.1.100 FabG1
-
 Haloferax volcanii
1.1.1.100 FabG1
-
 Haloarcula hispanica
1.1.1.100 PHA-specific acetoacetyl-CoA reductase
-
 Haloferax volcanii
1.1.1.100 PHA-specific acetoacetyl-CoA reductase
-
 Haloarcula hispanica
1.1.1.100 PhaB
-
 Haloferax volcanii
1.1.1.100 PhaB
-
 Haloarcula hispanica
1.1.1.100 polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase
-
 Haloferax volcanii
1.1.1.100 polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase
-
 Haloarcula hispanica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADPH
-
 Haloarcula hispanica
1.1.1.100 NADPH dependent on Haloarcula hispanica

General Information

EC Number General Information Comment Organism
1.1.1.100 malfunction DELTAfabG1 cells lose the ability to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate). When the DELTAfabG1 strain is transformed with the fabG1 expression plasmid pWLG1, the capability of polyhydroxyalkanoate accumulation is restored. When the fabG1 gene is disrupted or knocked out, the NADPH-dependent activity of acetoacetyl-CoA reductase in the mutant, the DELTAfabG1 or fabG1-1 strain, is greatly decreased Haloarcula hispanica
1.1.1.100 malfunction polyhydroxyalkanoate-defective haloarchaeon Haloferax volcanii
1.1.1.100 physiological function FabG1 has the function of supplying precursors for polyhydroxyalkanoate biosynthesis Haloarcula hispanica