Literature summary extracted from
Chen, H.J.; Pan, S.C.; Shaw, G.C.
Identification and characterization of a novel intracellular poly(3-hydroxybutyrate) depolymerase from Bacillus megaterium (2009), Appl. Environ. Microbiol., 75, 5290-5299.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.75 |
expressed as a His-tagged fusion protein in Escherichia coli |
Priestia megaterium |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.1.75 |
35740 |
- |
calculated from cDNA |
Priestia megaterium |
3.1.1.75 |
38000 |
- |
His-tagged fusion protein, SDS-PAGE |
Priestia megaterium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.75 |
Priestia megaterium |
C7A621 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.75 |
using Ni-NTA chromatography |
Priestia megaterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.75 |
poly(3-hydroxybutyrate) + H2O |
intracellular native poly(3-hydroxybutyrate) granules in vitro are rapidly degraded without the need for protease treatment. Almost all the final hydrolytic products produced from the in vitro degradation of nPHB granules by PhaZ1 are 3-hydroxybutyric acid monomers. PhaZ1 is also able to hydrolyze denatured semicrystalline poly(3-hydroxybutyrate), with the generation of 3-hydroxybutyric acid monomers |
Priestia megaterium |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.75 |
PhaZ1 |
- |
Priestia megaterium |
3.1.1.75 |
poly(3-hydroxybutyrate) depolymerase |
- |
Priestia megaterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.1.75 |
37 |
- |
assay at |
Priestia megaterium |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.1.75 |
8 |
- |
assay at |
Priestia megaterium |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.1.1.75 |
malfunction |
disruption of the phaZ1 gene significantly affects intracellular poly(3-hydroxybutyrate) mobilization during the poly(3-hydroxybutyrate)-degrading stage in Bacillus megaterium, as demonstrated by transmission electron microscopy and the measurement of the poly(3-hydroxybutyrate) content |
Priestia megaterium |