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Literature summary extracted from

  • Yeom, S.J.; Ji, J.H.; Kim, N.H.; Park, C.S.; Oh, D.K.
    Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose (2009), Appl. Environ. Microbiol., 75, 4705-4710.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.3.1.8 synthesis mannose-6-phosphate isomerase from Bacillus subtilis is applied for the production of L-ribose by direct isomerization of L-ribulose Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8 expression in Escherichia coli strain ER2566 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 EDTA complete inactivation Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.8 110
-
 D-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 312
-
 L-allose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 433
-
 D-Lyxose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 469
-
 D-talose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 946
-
 D-mannose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 998
-
 L-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.8 Ba2+ activates Bacillus subtilis
5.3.1.8 Ca2+ activates Bacillus subtilis
5.3.1.8 Co2+ activates, best metal ion, maximal activity at 0.5 mM Bacillus subtilis
5.3.1.8 Cu2+ activates Bacillus subtilis
5.3.1.8 Mg2+ activates Bacillus subtilis
5.3.1.8 Mn2+ highly activating Bacillus subtilis
5.3.1.8 additional information mannose-6-phosphate isomerases are metalloenzymes that require a divalent ion metal cofactor for activity and catalysis Bacillus subtilis
5.3.1.8 Ni2+ activates Bacillus subtilis
5.3.1.8 Zn2+ activates Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.8 36444
-
 * 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation Bacillus subtilis
5.3.1.8 36500
-
 * 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation Bacillus subtilis
5.3.1.8 36600
-
 gel filtration, recombinant enzyme Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate Bacillus subtilis
-
 D-Fructose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Bacillus subtilis Q9AGZ4 enzyme from Bacillus subtilis strain HB002; ATCC 23857
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.8 recombinant enzyme 27fold from Escherichia coli strain ER2566 by affinity chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.8 additional information
-
 substrate specificity, overview Bacillus subtilis
5.3.1.8 0.0101
-
 recombinant enzyme Bacillus subtilis
5.3.1.8 0.0165
-
 recombinant enzyme Bacillus subtilis
5.3.1.8 0.0225
-
 recombinant enzyme Bacillus subtilis
5.3.1.8 0.0659
-
 recombinant enzyme Bacillus subtilis
5.3.1.8 0.0918
-
 recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-allose
-
 Bacillus subtilis D-psicose
-
 r
5.3.1.8 D-Lyxose
-
 Bacillus subtilis D-Xylulose
-
 r
5.3.1.8 D-Mannose
-
 Bacillus subtilis D-Fructose
-
 r
5.3.1.8 D-Mannose 6-phosphate
-
 Bacillus subtilis D-Fructose 6-phosphate
-
 r
5.3.1.8 D-ribose
-
 Bacillus subtilis D-ribulose
-
 r
5.3.1.8 D-talose
-
 Bacillus subtilis D-tagatose
-
 r
5.3.1.8 L-allose
-
 Bacillus subtilis L-psicose
-
 r
5.3.1.8 L-lyxose
-
 Bacillus subtilis L-xylulose
-
 r
5.3.1.8 L-Mannose
-
 Bacillus subtilis L-Fructose
-
 r
5.3.1.8 L-ribose
-
 Bacillus subtilis L-ribulose
-
 r
5.3.1.8 L-ribulose best substrate Bacillus subtilis L-ribose
-
 r
5.3.1.8 L-talose
-
 Bacillus subtilis L-tagatose
-
 r
5.3.1.8 additional information the enzyme is specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the D- and L-enantiomers of ribose, lyxose, talose, mannose, and allose, substrate specificity, overview Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.8 monomer * 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.8 40
-
 recombinant enzyme Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.8 20 50
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.8 additional information
-
 first-order kinetics for thermal inactivation Bacillus subtilis
5.3.1.8 25
-
 purified recombinant enzyme, half-life is 461 h Bacillus subtilis
5.3.1.8 30
-
 purified recombinant enzyme, half-life is 325 h Bacillus subtilis
5.3.1.8 35
-
 purified recombinant enzyme, half-life is 236 h Bacillus subtilis
5.3.1.8 40
-
 purified recombinant enzyme, half-life is 111 h Bacillus subtilis
5.3.1.8 45
-
 purified recombinant enzyme, half-life is 56 h Bacillus subtilis
5.3.1.8 50
-
 purified recombinant enzyme, half-life is 10 h Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.8 72
-
 D-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 920
-
 L-allose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 3748
-
 D-mannose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 7089
-
 D-Lyxose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 17600
-
 L-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 63970
-
 D-talose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.8 7.5
-
 recombinant enzyme Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.8 6.5 8.5
-
 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.8 0.6
-
 D-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 2.9
-
 L-allose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 3.5
-
 D-mannose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 6.8
-
 D-talose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 16.4
-
 D-Lyxose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis
5.3.1.8 17.6
-
 L-ribose pH 7.5, 40°C, recombinant enzyme Bacillus subtilis