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Literature summary extracted from

  • Reilly, T.J.; Chance, D.L.; Calcutt, M.J.; Tanner, J.J.; Felts, R.L.; Waller, S.C.; Henzl, M.T.; Mawhinney, T.P.; Ganjam, I.K.; Fales, W.H.
    Characterization of a unique class C acid phosphatase from Clostridium perfringens (2009), Appl. Environ. Microbiol., 75, 3745-3754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 His-tagged version expressed in Escherichia coli BL21 AI Clostridium perfringens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 D100A 1% wild-type activity Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Cd2+
-
 Clostridium perfringens
3.1.3.2 Ce3+
-
 Clostridium perfringens
3.1.3.2 guanosine
-
 Clostridium perfringens
3.1.3.2 Hg2+
-
 Clostridium perfringens
3.1.3.2 molybdate
-
 Clostridium perfringens
3.1.3.2 NaF
-
 Clostridium perfringens
3.1.3.2 Pb2+
-
 Clostridium perfringens
3.1.3.2 ribose
-
 Clostridium perfringens
3.1.3.2 Sm2+
-
 Clostridium perfringens
3.1.3.2 Tartrate
-
 Clostridium perfringens
3.1.3.2 vanadate
-
 Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.221
-
 5'-UMP
-
 Clostridium perfringens
3.1.3.2 0.222
-
 5'-TMP
-
 Clostridium perfringens
3.1.3.2 0.269
-
 5'-AMP
-
 Clostridium perfringens
3.1.3.2 0.283
-
 5'-GMP
-
 Clostridium perfringens
3.1.3.2 0.658
-
 p-nitrophenyl phosphate
-
 Clostridium perfringens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Co2+
-
 Clostridium perfringens
3.1.3.2 Cr2+
-
 Clostridium perfringens
3.1.3.2 Cu2+
-
 Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 31000
-
 2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Clostridium perfringens B1Q002 ATCC 10543
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography Clostridium perfringens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.2
-
 Clostridium perfringens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 46.5
-
 substrate 5'-AMP Clostridium perfringens
3.1.3.2 47.4
-
 substrate 5'-TMP Clostridium perfringens
3.1.3.2 52.1
-
 substrate 5'-GMP Clostridium perfringens
3.1.3.2 71.4
-
 substrate 5'-UMP Clostridium perfringens
3.1.3.2 98.3
-
 substrate p-nitrophenyl phosphate Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3'-TMP + H2O
-
 Clostridium perfringens thymidine + phosphate
-
 ?
3.1.3.2 4-methylumbelliferyl phosphate + H2O
-
 Clostridium perfringens 4-methylumbelliferol + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Clostridium perfringens adenosine + phosphate
-
 ?
3.1.3.2 5'-GMP + H2O
-
 Clostridium perfringens guanosine + phosphate
-
 ?
3.1.3.2 5'-TMP + H2O
-
 Clostridium perfringens thymidine + phosphate
-
 ?
3.1.3.2 5'-UMP + H2O
-
 Clostridium perfringens uridine + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O further substrates: phenolphthalein bisphosphate, phenolphthalein monophosphate Clostridium perfringens p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 homodimer 2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE Clostridium perfringens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 class C acid phosphatase
-
 Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.6
-
 substrate p-nitrophenyl phosphate Clostridium perfringens
3.1.3.2 6
-
 substrate 5'-UMP Clostridium perfringens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.2 additional information
-
 80% inhibition at 1 mM Clostridium perfringens Pb2+
3.1.3.2 0.0012
-
-
 Clostridium perfringens Hg2+
3.1.3.2 0.5
-
-
 Clostridium perfringens NaF