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Literature summary extracted from

  • Somers, W.A.C.; Stigter, E.C.A.; Van Hartingsveldt, W.; Van Der Lugt, J.P.
    Enantioselective oxidation of secondary alcohols at a quinohemoprotein alcohol dehydrogenase electrode (1999), Appl. Biochem. Biotechnol., 75, 151-161.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.9.1 biotechnology co-immobilization of enzyme with redox polymer poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A per m2 depending on the substrate and the operation temperature. The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the (S)-2-alcohols, the enantioselectivity increases with increasing chain length. The enantiomeric ratio E increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.9.1 0.005
-
 1-butanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.005
-
 1-Pentanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.005
-
 1-Octanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.006
-
 1-Pentanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.006
-
 1-Octanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.006
-
 1-butanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.04
-
 (S)-2-heptanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.05
-
 2-Octanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.05
-
 (S)-2-octanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.05
-
 2-Octanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.05
-
 (S)-2-octanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.06
-
 1-propanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.06
-
 (S)-2-heptanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.06
-
 1-propanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.09
-
 (S)-2-hexanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.09
-
 (S)-2-hexanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.1
-
 (R)-solketal enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.11
-
 (R)-solketal soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.24
-
 5-methyl-2-hexanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.3
-
 1,3-butanediol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.4
-
 2-Pentanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.4
-
 (R)-2-octanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.4
-
 4-decanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.4
-
 4-decanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.6
-
 (R)-2-hexanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.6
-
 2-Pentanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.7
-
 4-heptanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.7
-
 (R)-2-heptanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.7
-
 4-heptanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.75
-
 4-methyl-2-pentanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.8
-
 Cyclohexanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.8
-
 3-nonanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.8
-
 3-nonanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.83
-
 Cyclohexanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.9
-
 3-octanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.9
-
 3-methyl-2-pentanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.9
-
 3-octanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 0.99
-
 (S)-solketal soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 1
-
 (S)-solketal enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 1.1
-
 (R)-2-heptanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 1.2
-
 (R)-2-hexanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 2.2
-
 ethanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 2.2
-
 ethanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 2.9
-
 3-Heptanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 4.3
-
 3-Heptanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 4.5
-
 (S)-glycidol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 4.5
-
 (S)-2-butanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 4.8
-
 (R)-glycidol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 5
-
 (S)-2-butanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 5.3
-
 3-Hexanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 6.3
-
 3-Hexanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 8
-
 2-butanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 12
-
 2-butanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 22
-
 2-propanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 28
-
 3-Pentanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 28
-
 (R)-2-butanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 30
-
 (R)-2-butanol enzyme electrode, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 30
-
 2-propanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni
1.1.9.1 30
-
 3-Pentanol soluble enzyme, pH 7.7, presence of CaCl2 Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.1.9.1 Comamonas testosteroni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.9.1 (R)-2-butanol + ferricyanide enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively Comamonas testosteroni butan-2-one + ferrocyanide
-
 ?
1.1.9.1 (R)-2-heptanol + ferricyanide enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively Comamonas testosteroni heptan-2-one + ferrocyanide
-
 ?
1.1.9.1 (R)-2-hexanol + ferrocyanide enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively Comamonas testosteroni hexan-2-one + ferricyanide
-
 ?
1.1.9.1 (R)-2-octanol + ferrocyanide enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively Comamonas testosteroni octan-2-one + ferricyanide
-
 ?
1.1.9.1 (R)-glycidol + ferrocyanide enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction Comamonas testosteroni ? + ferricyanide
-
 ?
1.1.9.1 (R)-solketal + ferrocyanide enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively Comamonas testosteroni ? + ferricyanide
-
 ?
1.1.9.1 (S)-2-butanol + ferricyanide enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively Comamonas testosteroni butan-2-one + ferrocyanide
-
 ?
1.1.9.1 (S)-2-heptanol + ferricyanide enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively Comamonas testosteroni heptan-2-one + ferrocyanide
-
 ?
1.1.9.1 (S)-2-hexanol + ferrocyanide enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively Comamonas testosteroni hexan-2-one + ferricyanide
-
 ?
1.1.9.1 (S)-2-octanol + ferrocyanide enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively Comamonas testosteroni octan-2-one + ferricyanide
-
 ?
1.1.9.1 (S)-glycidol + ferrocyanide enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction Comamonas testosteroni ? + ferricyanide
-
 ?
1.1.9.1 (S)-solketal + ferrocyanide enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively Comamonas testosteroni ? + ferricyanide
-
 ?
1.1.9.1 1,3-butanediol + ferricyanide enzyme electrode, 91% of vmax with 1-butanol Comamonas testosteroni ? + ferrocyanide
-
 ?
1.1.9.1 1-butanol + ferricyanide maximum velocity rate for both enzyme electrode and soluble enzyme Comamonas testosteroni butanal + ferrocyanide
-
 ?
1.1.9.1 1-octanol + ferrocyanide enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol Comamonas testosteroni octanal + ferricyanide
-
 ?
1.1.9.1 1-pentanol + ferrocyanide enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol Comamonas testosteroni pentanal + ferricyanide
-
 ?
1.1.9.1 1-propanol + ferricyanide enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 90% of vmax with 1-butanol Comamonas testosteroni propanal + ferrocyanide
-
 ?
1.1.9.1 2-butanol + ferrocyanide enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni butanone + ferricyanide
-
 ?
1.1.9.1 2-octanol + ferrocyanide enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol Comamonas testosteroni octan-2-one + ferricyanide
-
 ?
1.1.9.1 2-pentanol + ferrocyanide enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol Comamonas testosteroni pentan-2-one + ferricyanide
-
 ?
1.1.9.1 2-propanol + ferrocyanide enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol Comamonas testosteroni acetone + ferricyanide
-
 ?
1.1.9.1 3-heptanol + ferrocyanide enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol Comamonas testosteroni heptan-3-one + ferricyanide
-
 ?
1.1.9.1 3-hexanol + ferrocyanide enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol Comamonas testosteroni hexan-3-one + ferricyanide
-
 ?
1.1.9.1 3-methyl-2-pentanol + ferricyanide enzyme electrode, 3.7% of vmax with 1-butanol Comamonas testosteroni 3-methylpentan-2-one + ferrocyanide
-
 ?
1.1.9.1 3-methyl-2-pentanol + ferricyanide enzyme electrode, 9.3% of vmax with 1-butanol Comamonas testosteroni 3-methylpentan-2-one + ferrocyanide
-
 ?
1.1.9.1 3-nonanol + ferricyanide enzyme electrode, 4.7% of vmax with 1-butanol, soluble enzyme, 4.5% of vmax with 1-butanol Comamonas testosteroni nonan-3-one + ferrocyanide
-
 ?
1.1.9.1 3-octanol + ferrocyanide enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni octan-3-one + ferricyanide
-
 ?
1.1.9.1 3-pentanol + ferrocyanide enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol Comamonas testosteroni pentan-3-one + ferricyanide
-
 ?
1.1.9.1 4-decanol + ferricyanide enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 3.6% of vmax with 1-butanol Comamonas testosteroni decan-4-one + ferrocyanide
-
 ?
1.1.9.1 4-heptanol + ferricyanide enzyme electrode, 9.3% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni heptan-4-one + ferrocyanide
-
 ?
1.1.9.1 4-methyl-2-pentanol + ferricyanide enzyme electrode, 5.6% of vmax with 1-butanol Comamonas testosteroni 4-methylpentan-2-one + ferrocyanide
-
 ?
1.1.9.1 5-methyl-2-hexanol + ferricyanide
-
 Comamonas testosteroni 5-methylhexan-2-one + ferrocyanide
-
 r
1.1.9.1 cyclohexanol + ferricyanide enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol Comamonas testosteroni cyclohexanone + ferrocyanide
-
 ?
1.1.9.1 ethanol + ferricyanide 86% of vmax with 1-butanol, soluble enzyme, 75% of vmax with 1-butanol Comamonas testosteroni ethanal + ferrocyanide
-
 ?
1.1.9.1 additional information no substrate: D-glucose, D-galactose, D-fructose, Alpha-D-methylglucoside, mannitol Comamonas testosteroni ?
-
 ?
1.1.9.1 additional information The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate. The same property is observed for secondary alcohols in the series 2-propanol to 2-octanol Comamonas testosteroni ?
-
 ?