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Literature summary extracted from

  • Heinemann, I.U.; Schulz, C.; Schubert, W.D.; Heinz, D.W.; Wang, Y.G.; Kobayashi, Y.; Awa, Y.; Wachi, M.; Jahn, D.; Jahn, M.
    Structure of the heme biosynthetic Pseudomonas aeruginosa porphobilinogen synthase in complex with the antibiotic alaremycin (2010), Antimicrob. Agents Chemother., 54, 267-272.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.24 expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.24 porphobilinogen synthase is cocrystallized with the alaremycin Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.24 alaremycin porphobilinogen synthase is cocrystallized with the alaremycin. At 1.75 A resolution, the crystal structure reveals that the antibiotic efficiently blocks the active site of porphobilinogen synthase. The antibiotic binds as a reduced derivative of 5-acetamido-4-oxo-5-hexenoic acid. The corresponding methyl group is not coordinated by any amino acid residues of the active site, excluding its functional relevance for alaremycin inhibition. Alaremycin is covalently bound by the catalytically important active-site lysine residue 260 and is tightly coordinated by several active-site amino acids Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.24 0.00032
-
 5-aminolevulinic acid
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.24 Pseudomonas aeruginosa Q59643
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.24 using affinity chromatography and anion-exchange chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.24 5-aminolevulinic acid + 5-aminolevulinic acid
-
 Pseudomonas aeruginosa porphobilinogen + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.24 Porphobilinogen synthase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.24 16.1
-
 5-aminolevulinic acid
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.24 1.33
-
 alaremycin
-
 Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.24 1.33
-
-
 Pseudomonas aeruginosa alaremycin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.24 50300
-
 5-aminolevulinic acid
-
 Pseudomonas aeruginosa