BRENDA - Enzyme Database

AAC(6)-Iaf, a novel aminoglycoside 6-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates

Kitao, T.; Miyoshi-Akiyama, T.; Kirikae, T.; Antimicrob. Agents Chemother. 53, 2327-2334 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.82
into the vectors pSTV28 and pQE2, pQE2 is used for expression of the protein in Escherichia coli BL21DE3 pLysS cells
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
21000
-
determined by SDS-PAGE and Western Blot analysis
Pseudomonas aeruginosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.82
Pseudomonas aeruginosa
C4TGI5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.82
recombinant AAC(6')-Iaf is purified using Ni-nitrilotriacetic acid agarose, native AAC(6')-Iaf from Pseudomonas aeruginosa on an anti-AAC(6')-Iaf-IgG coupled and NHS-activated Sepharose column
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + gentamicin
-
701732
Pseudomonas aeruginosa
CoA + 6'-N-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin
-
701732
Pseudomonas aeruginosa
CoA + N6'-acetylneomycin
-
-
-
?
2.3.1.82
additional information
lividomycin A is also a substrate, although with extremely small activity, thus the enzyme has acetylation activity with alternate amino groups in aminoglycosides
701732
Pseudomonas aeruginosa
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.82
acetyl-CoA
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
into the vectors pSTV28 and pQE2, pQE2 is used for expression of the protein in Escherichia coli BL21DE3 pLysS cells
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.82
acetyl-CoA
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
21000
-
determined by SDS-PAGE and Western Blot analysis
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
recombinant AAC(6')-Iaf is purified using Ni-nitrilotriacetic acid agarose, native AAC(6')-Iaf from Pseudomonas aeruginosa on an anti-AAC(6')-Iaf-IgG coupled and NHS-activated Sepharose column
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + gentamicin
-
701732
Pseudomonas aeruginosa
CoA + 6'-N-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin
-
701732
Pseudomonas aeruginosa
CoA + N6'-acetylneomycin
-
-
-
?
2.3.1.82
additional information
lividomycin A is also a substrate, although with extremely small activity, thus the enzyme has acetylation activity with alternate amino groups in aminoglycosides
701732
Pseudomonas aeruginosa
?
-
-
-
-