EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.99.4 | medicine | alpha-methylacyl-coenzyme A racemase is a biomarker for prostate cancer and a putative target for the development of therapeutic agents directed against the disease, inhibition of enzyme activity may also act synergistically with androgen ablation therapy | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.99.4 | the open reading frame encoding alpha-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis is subcloned into a pET15b vector, and the enzyme is overexpressed in Escherichia coli cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.99.4 | (2R)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis | |
5.1.99.4 | (2S)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis | |
5.1.99.4 | octyl-beta-D-glucopyranoside | concentrations greater than 0.6% octyl-beta-D-glucopyranoside cause a reduction in enzyme activity | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.99.4 | 0.048 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.071 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.086 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.087 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.1.99.4 | peroxisome | - |
Mycobacterium tuberculosis | 5777 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.99.4 | 38970 | - |
calculated for recombinant enzyme without six-His tag | Mycobacterium tuberculosis |
5.1.99.4 | 40850 | - |
calculated for recombinant enzyme with six-His tag | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.99.4 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.99.4 | by using metal ion affinity chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.99.4 | (2R)-ibuprofenoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-ibuprofenoyl-CoA | - |
r | |
5.1.99.4 | (2R)-pristanoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-pristanoyl-CoA | - |
r | |
5.1.99.4 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA | - |
Mycobacterium tuberculosis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.99.4 | alpha-methylacyl-coenzyme A racemase | - |
Mycobacterium tuberculosis |
5.1.99.4 | AMACR | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.99.4 | 228 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 291 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 358 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 450 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.1.99.4 | metabolism | beta-oxidation pathway of fatty acids | Mycobacterium tuberculosis |
5.1.99.4 | physiological function | enzyme catalyzes the 1,1-proton transfer reaction that effects the reversible epimerization of the coenzyme A thioesters derived from a variety of (2R)- and (2S)-methyl branched fatty acids | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.99.4 | additional information | - |
additional information | Mycobacterium tuberculosis enzyme expressed as a fusion protein bearing an N-terminal six-His tag has a catalytic efficiency that is reduced 22% and 47% in the 2S-2R and 2R-2S directions, respectively, relative to untagged enzyme | Mycobacterium tuberculosis | |
5.1.99.4 | 0.0032 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.0041 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.0052 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
5.1.99.4 | 0.0061 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |