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Literature summary extracted from
- Enzymatic recovery of platinum (IV) from industrial wastewater using a biosulphidogenic hydrogenase (2008), Afr. J. Biotechnol., 7, 1087-1095.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.12.2.1 | industry | platinum (IV) recovery from an industrial effluent through a biosulphidogenic sulphate reducing bacteria consortium via a hydrogenase-cytochrome c3 enzyme system that removes electrons from hydrogen to the platinum metal that acts as a final electron acceptor | Bacteria |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.2.1 | Ag+ | 1 mM, 97% inhibition | Bacteria |  |
| 1.12.2.1 | Cr6+ | 1 mM, complete inhibition | Bacteria | |
| 1.12.2.1 | Cu2+ | 1 mM, 95% inhibition | Bacteria | |
| 1.12.2.1 | Zn2+ | 1 mM, 79% inhibition | Bacteria | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.12.2.1 | 0.0052 | - |
methyl viologen | - |
Bacteria | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.12.2.1 | membrane | - |
Bacteria | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.2.1 | Iron | increases activity by 1.8% | Bacteria | |
| 1.12.2.1 | Nickel | increases activity by 1.6% | Bacteria | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.2.1 | 58000 | - |
x * 58000, SDS-PAGE | Bacteria |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.2.1 | Bacteria | - |
sulphate reducing bacteria | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.2.1 | by sonication, PEG 20000 concentration, ion exchange chromatography and gel filtration, 9.26fold purified with a yield of 51.8% | Bacteria |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.2.1 | 3817 | - |
crude sonicate | Bacteria |
| 1.12.2.1 | 35340 | - |
9.26fold purified | Bacteria |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.2.1 | ferricytochrome c3 + 2 H2 | 48% relative activity compared to activity with oxidized methyl viologen | Bacteria | ferrocytochrome c3 + 4 H+ | - |
? | |
| 1.12.2.1 | H2 + oxidized dichloroindophenol | 30% relative activity compared to activity with oxidized methyl viologen | Bacteria | H+ + ? | - |
? | |
| 1.12.2.1 | H2 + oxidized methyl viologen | 100% relative activity | Bacteria | H+ + reduced methyl viologen | - |
? | |
| 1.12.2.1 | H2 + potassium ferricyanide | 34% relative activity compared to activity with oxidized methyl viologen | Bacteria | H+ + ? | - |
? | |
| 1.12.2.1 | additional information | hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3 | Bacteria | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.12.2.1 | ? | x * 58000, SDS-PAGE | Bacteria |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.2.1 | 35 | 40 | - |
Bacteria |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.2.1 | 35 | 40 | at optimal temperature range of 35-40°C, a minimal activity loss during the first 10 to 20 min and thereafter, activity steadily decreases as incubation time increases. After 80 min of incubation, the enzyme loses 75-80% of all of its activity | Bacteria |
| 1.12.2.1 | 55 | - |
completely denatured above | Bacteria |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.12.2.1 | 3.6 | - |
methyl viologen | - |
Bacteria | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.2.1 | 7.5 | 8 | - |
Bacteria |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.2.1 | cytochrome c3 | hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3. Reduction of platinum by the purified hydrogenase enzyme alone yields about 10% removal after 3 h incubation while after the addition of cytochrome c3 there is a slight increase to 15%. Suppression of enzyme activity due to the extreme acid pH of 0.38 of the effluent | Bacteria |
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Release 2023.1 (January 2023)
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